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Abstract
This study examines the effective concentration of temu kunci extract (Bosenbergia pandurata) as an antibacterial inhibition zone against 3 Aerococcus sp. isolates. and 2 Streptococus sp. isolates. The method used is the ADD (Agar Diffusion Disc) method and bacterial culture together with the bacterial count using the TPC (Total Plate Count) method. Extract concentrations used were concentrations of 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm, 600 ppm, 700 ppm, 800 ppm, 900 ppm, and 1000 ppm. Positive controls used were amoxicillin concentration of 500 ppm and negative controls using sterile aquades. Bacteria were grown on BHIB media and then as much as 1 ml were spread on BHIA media on petri dishes, then 20 μl drops were dropped on an antibacterial disc measuring 6 mm, then incubated at 30ºC for 48 hours and carried out also for 24 hours and 48 hours. Furthermore, in the second experiment carried out by culture together the extract concentrations used were 600 and 800 ppm, the results showed that the concentration of 800 ppm was greater than the growth of bacterial destruction by the ADD method and the TPC method and the zone of inhibition of the extract against the test bacteria 10.61- 13.47 mm.
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References
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References
Chahyadi, A, Hartati, R., Wirasutisna, K. R., & Elfahmi. 2014. Boesenbergiapandurata Roxb, An Indonesian Medicinal Plant : Phytochemistry, Biological Activity, Plant Biotechnology.Procedia Chemistry, 13-37
Erviani, A. E. 2013. Analisi Multidrug Resistensi terhadap Antibiotik pada Salmonella typhi dengan Teknik Mulplex PCR.biogenesis, 51-60
Fardiaz. 2004. Analisa Mikrobiologi Pangan. PT. Raja Grafindo Persada : Jakarta.
Hardi, E.H, Sukenda, E. Harris, dan A.M Lusiastuti. 2011. Karakteristik dan Patogenisitas Streptococcus Agalactiae Tipe β β β β β-hemolitik dan Non-hemolitik pada Ikan Nila. Jurnal Veteriner. Vol. 12 No. 2: 152-164
Hardi, E.H, W.K Irawan, Suwinarti W, Agustina, dan A.N Rudi. 2016. Antibacterial activity of Boesenbergia pandurata, Zingiber zerumbet and Solanum ferox extracts againts Aeromonas hydrophilla and Pseudomonas sp. Jurnal Veteriner. 8 (No.)1:18-21
Hardi, E.H, G. Septiani, R.A. Nugroho, R. Sarinah dan M. Agriandini. 2018. Identification of Potential Pathogenic Bacteria from Tilapia (Oreochromis niloticus) and Channel Catfish (Clarias batracus) Culture in Samarinda East Kalimantan. Biodiversitas, Inpress.
Jaksa, S. 2009. Minyak Atsiri Dari Beberapa Tanaman Obat.Jurnal Kedokterandan Kesehatan.
Lewbart. 2006. "Crustaceans". Invertebrate Medicine. Wiley-Blackwell. pp. 179–194. ISBN 978-0-8138-1844-3.
Miksusanti, Jennie, B. S., Ponco, B., & Trimulyadi, G. 2008. Kerusakan Dinding Sel Eschericia coli kl.l oleh Minyak Atsiri Temu Kunci (Kaempferiapandurata). Berita Biologi Jurnal Ilmiah Nasional, 1-8.
Saptiani,G., S. B. Prayitno dan S. Anggoro. 2012. Efektifitas Ekstrak Daun Jeruju (Acanthus ilicifolius) Melindungi Udang Windu (Penaeus monodon F.) dari Infeksi Vibrio harveyi. J. of Coastal Development, 15(2): 217–224
Uderwood J.C.E . 1999.Karakteristik, Klasifikasi dan insiden Penyakit , patologi umum dan sistemik, edisi 2. buku kedokteran: Jakarta Waluyo, Lud, 2005. Mikrobiologi Umum. Malang : UMM Press.
Zuhud, EAM., Rahayu, WP., Wijaya, CH., dan Sari, PP. 2001. Aktivitas Antimikroba Ekstrak Kedawung (Parkia roxburghii G. Don) terhadap Bakteri Patogen.Jurnal Teknologi dan Industri