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Abstract

Tilapia (Oreochromis niloticus) is one of the leading freshwater fish commodities in Indonesia. The problem often faced by tilapia farmers is the low level of feed utilization, especially protein, which is still low, resulting in suboptimal growth. The glutamate content in monosodium glutamate (MSG) can act as a precursor for the formation of arginine and glutamine, which can increase the production of protease and lipase enzymes that are excreted into the intestines during the feed hydrolysis process, which is useful in increasing the absorption of nutrients in the form of protein and fat. This research was conducted using fingerling tilapia as a test fish with an average individual length of 6.59 ± 0.21 cm/fish and an average individual weight of 5.33 ± 0.27 g/fish. This research was carried out in an aquarium container measuring 40x30x30 cm with a water volume of 27 l and a density of 15 fish/aquarium. The aim of this research was to determine the effect of MSG in fish feed on total feed consumption (TKP), feed utilization efficiency (FUE), protein efficiency ratio (PER), protein retention, relative growth rate (RGR), and survival (SR) in fingerling tilapia. This research used an experimental method with a completely randomized design (CRD) of 4 treatments with 3 replications. Treatments A, B, C, and D are fed with the addition of 0 MSG in commercial feed; 0.75; 1.5; and 2.25 g/kg. Variables observed included TKP, FUE, PER, protein retention, RGR, and SR. The research results showed that MSG in feed had a significant effect (P < 0.05) on the FUE, PER, protein retention, and RGR values but had no effect (P < 0.05) on the TKP and SR values. FUE value; PER, protein retention; The highest RGR was obtained in treatment D (2.25 g/kg); FUE (87.21±2.28); PER(1.83±0.05); protein retention (50.47±0.76); The RGR (3.06 ± 0.03) and survival value for all treatments were found to be 100%. The water quality during the research was in optimal condition.

Keywords

Tilapia (Oreochromis niloticus), Monosodium Glutamate, Growth Parameters

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Copyright (c) 2023 Aquacultura Indonesiana

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Author Biographies

Dyah Ayu Indriati, Diponegoro University

Department of Aquaculture

Subandiyono Subandiyono, Diponegoro University

Lecturer of Aquaculture Departemen, Faculty Fisheries and Marine Science, Diponegoro University

Seto Windarto, Diponegoro University

Lecturer of Aquaculture Department, Faculty of Fisheries and Marine Science, Diponegoro University

References

  1. Afrianto, E., and Liviawaty, E. (2005). Fish Feed. Kanisius, Yogyakarta.
  2. Akbar, C., Utomo, DSC, Hudaidah, S., and Setyawan, A. 2020. Management of time and amount of feeding to increase growth and survival of snakehead fish, Channa striata (Bloch, 1793). Journal of Aquatropica Asia, 5(1): 1-8.
  3. Amalia, R. Subandiyono, and E. Arini. 2013. Effect of Papain Use on the Level of Feed Protein Utilization and Growth of Dumbo Catfish (Clarias gariepinus). Journal of Aquaculture Management and Technology,2(1): 136-143.
  4. Amarwati, H., Subandiyono, and Pinandoyo, 2015. The Effect of Dietary Fermentation Cassava Leaf Meal (Manihot utilissima) on the Artificial Feeds on the Growth Rate of Red Tilapia (Oreochromis niloticus) Seeds, Journal of Aquaculture
  5. Amri, K. 2021. Use of Probiotics in Containers for Raising Tilapia Seeds (Oreochromis niloticus) as water quality control. Arwana: Journal Scientific Aquatic Studies Program, 3(2): 141–149.
  6. Andriani, Y. 2018. Tilapia Fish Cultivation. Deepublish. Andriani, Y.; Setiawati, M.; and Sunarno, MTD 2019. Diet digestibility and growth performance of giant gouramy juveniles, Osphronemus goramy feed on diet
  7. supplemented with glutamine. Indonesian Journal of Ichthyology, 19(1):1–11.
  8. Arfiati, D., Farkha, K., and Anugerah, DP 2022. Tilapia fish (Oreochromis niloticus). Media Nusa Creative (MNC Publishing).
  9. Ariffianti, I., Amrul, R., Samudra, H., Setiawati, E., and Widowati, W. 2022. Rise with the tilapia fish farming business during the pandemic. Valid Journal Devotion, 1(1): 10–19.
  10. Arifin, OZ, Kurniasih, T., and Nugroho, E. 2017. Production of Super Homogametic (YY) male fish in Tilapia (Oreochromis niloticus). Indonesian Fisheries Research Journal, 10(1): 79–82.
  11. Arifin, MY (2017). Growth and Survival Rate of Tilapia (Oreochromis. sp.) Red Strain and Black Strain Maintained in Salinity Media. Jambi Batanghari University Scientific Journal, 16(1): 159–166.
  12. Asmaini, A., Handayani, L., and Nurhayati, N. 2020. Addition of Nano CaO from Kijing Shell (Pilsbryocncha exilis) Waste to Salinity Media for Growth of Tilapia Fish (Oreochromis niloticus). Acta Aquatica: Aquatic Sciences Journal, 7(1): 1–7.
  13. Astuti, P. (2018). Veterinary Endocrinology, UGM Press.
  14. Badriyah, L. 2019. Education on the Use of Monosodium Glutamate (MSG) in Food and its Effects on Health. Journal of Community Engagement and Empowerment, 1(2): 84–87.
  15. Barrows, FT, and RW Hardy, 2001. Nutrition and Feeding, in Wedemeyers, D. Fish hatchery management. Second edition, American Fisheries Society, Bethesda, Maryland, 483–558.
  16. Ballestrazzi RD, Lannari ED'agoro, and Mion A. 1994. The Effect of Dietary Protein Level and Source of Growth and Body Composition, Total Ammonia, and Relative Phosphate Excretion of Growing Sea Bass Dicentrarchuss Labrax. Aquaculture, 127: 197–206.
  17. Brosnan JT, Brosnan ME 2013. Glutamate is a truly functional amino acid. Amino Acids, 45(3): 413–418.
  18. BSN (National Standards Agency). 2009. Tilapia (Oreochromis niloticus Bleeker Production). Seeds for Culture. SNI 7550:2009. 12 p.
  19. Boyd, CE (1990). Water Quality Management for Pondo Fish Culture. Scientific Publishing Company, Amsterdam, Nederland.
  20. Cahyadi, U., Jusadi, D., Fauzi, IA, and Sunarma, A. 2020. Supplemental enzymes in Artificial Diets for North African Catfish Larvae Clarias gariepinus Burchell, 1822. Indonesian Journal of Ichthyology, 20(2): 155–169.
  21. Cheng, Z., DM Gatlin, and A. Buentello. 2012. Dietary Supplementation with Arginine and/or Glutamine Influences Growth Performance, Immune Responses, and Intestinal Morphology of Hybrid Striped Bass (Morone chrysops x Morone)
  22. saxatilis). Aquaculture. 362(1): 39–43.
  23. Clara, C. 2017. Evaluation of the Addition of Glutamic Acid and Monosodium Glutamate in Feed on the Growth Performance of Goldfish Cyprinus Carpio. Indonesian Journal of Ichthyology, 17(1): 1-23.
  24. Darwis, Mudeng, JD, and Londong, SNJ 2019. Aquaponic culture system of carp, Cyprinus carpio, at different stocking densities. Journal Aquaculture, 7(2): 15–21.
  25. Devani, V. (2020). Optimizing the Nutrient Composition of Artificial Fish Feed by using fuzzy linear programming. Industrial Engineering Journal: Research Results and Scientific Work in the Field of Industrial Engineering, 5(1): 20–26
  26. Dewi, P., Habibah, NA, Mustikaningtyas, D., Iswari, RS, Nugrahaningsih, WH, Marianti, A., and Christijanti, W. 2022. Potential of Active Compounds from Natural Ingredients. UNISMA PRESS.
  27. Didin Fatihudin, SE, Wikanta, W., Hammadi Fauzi, SP, and Firmansyah, MA 2023. Salt Farmers' Financial Literacy: for access to capital, productivity, and market information. UM Surabaya Publishing.
  28. Effendie, 1997. Fisheries Biology. Nusatama Library Foundation, Yogyakarta. 163.
  29. Elvince, R., and Veronica, E. 2021. Food habits of Baung fish (Mystus nemurus), Lais Baji (Kryptopterus palembangensis), and Saluang Balu
  30. (Rasbora argyrotaenia) in Lake Batu, Central Kalimantan. BAWAL Widya Capture Fisheries Research, 13(3): 133–143.
  31. Endraswari, LPMD; Cokrowati, N.; and Lumbessy, SY 2021. Fortification of Fish Feed with Gracilaria Sp. Seaweed Meal. in Cultivating Tilapia (Oreochromis niloticus). Marine Journal: Indonesian Journal of Marine Science and Technology, 14(1): 70–81.
  32. ES, EY, and Susilo, A. 2003. Study of the Nutrient Content of Mushroom White Oyster (Pleurotus ostreatus): Growing Media Waste for Ruminant Animal Feed. Journal Mathematics of Science and Technology, 4(1), 54–61.
  33. Gao Y, Lu S, Wu M, Yao W, Jin Z, and Wu X. 2019. Effects of dietary protein levels on growth, feed utilization, and expression of growth-related genes in juvenile giants grouper (Epinephelus lanceolatus). Aquaculture, 504:369–374.
  34. Gu, M., N. Bai, B. Xu, X. Xu, Q. Jia, and Z. Zhang. 2017. Protective Effect of Glutamine and Arginine Against Soybean Meal-Induced Enteritis in the Juvenile turbot (Scophthalmus maximus). Fish Shellfish Immunol. 70(1): 95-105.
  35. Hadinoto, S., and Idrus, S. (2018). Proportions and Contents of Proximate Body Parts of FishYellow-tail tuna (Thunnus albacares) from Maluku waters.
  36. Kemenperin: Biam Magazine, 14(2): 51–57.
  37. Hapsari, BM, Hutabarat, J., and Harwanto, D. 2020. Water Quality Performance, Growth and Survival of Tilapia (Oreochromis niloticus) in Aquaponic Systems with Different Types of Plants. Science of Tropical Aquaculture: Indonesian Journal of Tropical Aquaculture, 4(1):78–89.
  38. Hendry, W., and Prayitno, Y. 2021. The Effect of Feed Efficiency on the Growth and Survival of Tilapia (Oreocromis niloticus). Journal of Fisheries and Marine Research, 5(3): 634–645.
  39. Henggu, KU, and Nurdiansyah, Y. 2021. Review of Carbohydrate, Lipid, protein, and nucleic acid metabolism QUIMICA: Journal of Scientific and
  40. Applied Chemistry, 3(2), 9–17.
  41. Henry-Unaeze HN 2017. Update on the food safety of monosodium L-glutamate (MSG). Pathophysiology, 24(4):243-249.
  42. Herawati, VE, Pinandoyo, P., Darmanto, YS, Rismaningsih, N., Windarto, S., and Radjasa, OK, 2020. The effect of fermented duckweed (Lemna minor) in feed on the growth and nutritional quality of tilapia (Oreochromis niloticus). Biodiversity Journal of Biological Diversity, 21(7): 3350–3358.
  43. Hu K, Zhang JX, Feng L, Jiang WD, Wu P, Liu Y, Jiang J, and Zhou XQ (2015) Effect of dietary glutamine on growth performance, nonspecific immunity, and expression of cytokine genes, phosphorylation of the target of rapamycin (TOR), and antioxidative system in the spleen and head kidney of Jian carp (Cyprinus carpio var. Jian). Fish Physiol Biochem, 41:635–649.
  44. Husna, M., and SN Kurniawan. 2017. Role of Mammalian Targets of Rapamycin in Epilepsy. Neurona. 34(1):244-251.
  45. Indriati, PA, and Hafiludin, H.2022. Water Quality Management in Fish Hatcheries
  46. Tilapia (Oreochromis niloticus) at the East Teja Fish Seed Center in Pamekasan. Juvenil, Marine and Fisheries Scientific Journal, 3(2): 27–31.
  47. Irmawati, SP, Budi, DS, Pi, S., Larekeng, ISH, SP, M., Kadriah, IAK, and Iswanto, SH 2022. Production Technology for Male Monosex Tilapia. Nas Library Media.
  48. Jiang. JD Shi, XQ Zhou, L. Yin, L. Feng, Y. Liu, W.D. Jiang, and Y. Zhao. 2015. Effects of Glutamate on Growth, Antioxidant Capacity, and Antioxidant-Related Signaling Molecule Expression in Primary Cultures of Fish Enterocytes. Fish Physiol Biochem. 41(1): 1143–1153.
  49. Jullianty, I., Yulianto, T., and Miranti, S. 2020. Effect of Adding Yeast Saccharomyces cerevisiae to Feed on the Growth of Fish Seed Pomfret Trachinotus Blochii. Aquaculture Intek, 4(1): 44–57.
  50. Khalida, A.; Agustono, NIDN; and Lokapirnasari, WP 2017. Addition of lysine to commercial feed on the protein retention and energy retention of freshwater pomfret fish (Colossoma macropomum). Scientific Journal of Fisheries and Marine Affairs, 9(2): 98–106.
  51. Khasani, I. 2013. Attractants in Fish Feed: Types, Functions, and Responses of Fish. Media Aquaculture, 8(2): 127–133.
  52. Kurniasih, T., Jusadi, D., Suprayudi, MA, Nuryati, S., Zairin Jr., M., and Supriyono, E. 2020. Physiological Response and Growth Performance of Tilapia in Medium Low in ammonia and given glutamic acid supplements. Journal of Research
  53. Aquaculture, 15(3): 175–183.
  54. Kurniawati, A., M. Setiawati, D. Jusadi, and S. Eddy. 2019. Effect of Glutamic Acid Supplementation in Feed on the Growth Performance of Patin Fish, Pangasius hypophthalmus, on Different Ammonia Maintenance Media. {Thesis}. Postgraduate School, Bogor Agricultural Institute, Bogor, 105.
  55. Kurtanty Dien., Daeng, MF, Nurhidayat, PU 2018. Review of Monosodium Glutamate: How to Understand It Properly. Jakarta: Primary Fish Doctors Cooperative Indonesia.
  56. Laili, NA; Istyadji, M.; and Yulinda, R. 2022. The Effect of Natural Feed Mixtures (Artemia and Duck Egg Yolk) on Growth and Survival of Koi Fish Larvae (Cyprinus carpio). Journal of Science and Applications, 1(3): 143–149.
  57. Lante, S., Usman, and A. Laining (2015) Effect of Feed Protein Levels on the Growth and Survival of Black Tiger Prawns (Paneus monodon). Transfection Fab. Gadjah Mada University. J. Fisheries. 1(1):10–17.
  58. Lestari, PK; Cinnawara, HT; Patahiruddin, P.; and Muchlis, AMR 2023. Effect of Different Feeding on Absolute Growth and Nutritional Content of Tilapia (Oreochromis niloticus). Eucheuma Journal of Aquaculture, 1(1), 1–8.
  59. Li P., KS Mai, J. Trushenski, and G. Wu. 2009. New Developments in Fish Amino Acid Nutrition: Towards Functional and Environmentally Oriented Aquafeeds.Amino Acids, 3(7): 43–53.
  60. Li Y, Gong Y, Li Y, He D, Wu Y, Wang H. 2018. Sleep disturbances and psychological Distress is associated with functional dyspepsia. Rome III criteria.BMC Psychiatry, 18(1): 1–7.
  61. Maulianawati, D., & Lembang, MS 2022. Aquaculture water quality Syiah Kuala University Press.
  62. Mahfudz, LD, Bachelor, TA, and Sarengat, W. (2010). Efficiency of Protein Use Rations Containing Alcoholic Beverage Distillation Waste by Male Quail (Coturnix coturnix Japonica). Journal of Technology Animal Husbandry and Veterinary. Livestock Research and Development Center,887-894
  63. Manik, RRDS, and Arleston, J. 2021. Fish nutrition and feed. CV Widina Media Utama.
  64. Marzuqi, M., NW Astuti, and K. Suwirya. 2012. The Effect of Protein Levels and Feeding Ratios on the Growth of Tiger Grouper (Epinephelus fuscoguttatus). BBPP Gondol Marine Aquaculture, Bali. 4(1): 55–65.
  65. Maulidin, R., Z., A. Muchlisin, and AA Muhammadar. 2016. Growth and Feed Utilization of Snakehead Fish (Channa striata) at Different Papain Enzyme Concentrations. Maritime and Fisheries Student Scientific Journal Unsyiah. 1(3): 280–290.
  66. Melisa, M., Hasri, I., Arisa, II, Paridah, I., and Puspidayani, D. 2023. Parasite Investment Black Tilapia (Oreochromis niloticus) Seeds at UPTD BBI Lukup Rhino. MAHSEER: Journal of Aquatic and Fishery Sciences, 5(1): 43–51.
  67. National Research Council (NRC), 1997. Nutrient Requirements of Warm Water Fishes. National Acad. Press, Washington, DC, 276
  68. Negara, GAWP; Pinandoyo, P.; Herawati, VE; and Syakirin, MB 2022. Efficiency of Adding Anting Spinach Flour (Acalypha indica) and Waste Bean sprouts (Vigna radiata) to gourami (Osphronemus) (fish-fed gouramy).Pena Aquatics: Scientific Journal of Fisheries and Marine Affairs, 21(2):29–42.
  69. Ngaddi, A., Jusadi, D., Wasjan, W., and Supriyono, E. 2019. Evaluation of monosodium glutamate supplementation on physiological response, growth performance, and feed utilization in North African catfish Clarias gariepinus (Burchell, 1822). Indonesian Journal of Ichthyology, 19(3), 337–348
  70. Ni'matulloh, MA; Rejeki, S.; and Aryati, RW 2018. The Effect of Differences in Grading Frequency on the Growth and Survival of Siamese Catfish Larvae (Pangasianodon hypophthalmus). Tropical Aquaculture Science: Indonesian Journal of Tropical Aquaculture, 2(1): 20–29.
  71. Nurfitasari, I., Palupi, IF, Sari, CO, Munawaroh, S., Yuniarti, NN, and Ujiwisata, T. 2020. Response of tilapia fish digestibility to various types of feed. Nectar: Journal of Biology Education, 1(2): 21–28.
  72. Nurhayati, T., Wirayudha, RH, and Suptijah, P. 2022. Application and Characteristics Chemistry of Peptone from Eel Fish (Angulilla bicolor) Viscera Waste as Nutrients for bacterial growth. Journal of Fisheries Product Processing Indonesia, 25(3): 1–12.
  73. Nurchayati, S., Haeruddin, H., Basuki, F., and Sarjito, S. 2021. Suitability Analysis Saline Tilapia Cultivation Land (Oreochromis niloticus) in Aquaculture Tayu District (Analysis on Land Suitability and Cultivation of Saline Tilapia) (Oreochromis niloticus) at The Pond in Tayu District. Fisheries Science and Technology: Indonesian Journal of Fisheries Science and Technology, 17(4), 224–233.
  74. Nurlim, R. 2022. Textbook of Animal Physiology.
  75. NRC (National Research Council), 1993. Nutrient Requirements of Fish. Wangshington D.C.: National Academic Press, 115 pp.
  76. Ondang, HMP, Tumanduk, NM, Triyastuti, MS, Rakhmayeni, DA, Kaligis, DD, and Wowiling, F. 2022. Chemical and organoleptic characteristics of
  77. Squid Ink Flavoring with the Oven Method. Bluefin Fisheries Journal, 4(1): 17–26.
  78. Patmawati, H., Wahyuningsih, S., Mansyur, MZ, Yulianto, E., and Thibyan, M. 2023.Cultivation of Three Varieties of Tilapia (Oreochromis niloticus) in a Round Pool with a biofloc system. ABDINUS Journal: Journal of Indonesian Service, 7(1): 173–181.
  79. Pereira RT, Rosa PV, and Gatlin DM III (2017) Glutamine and arginine in diets for Nile tilapia: effects on growth, innate immune responses, plasma amino acid profile and whole-body composition. Aquaculture, 473:135–144.
  80. Pereira, L., T. Riquelme, and H. Hosokawa, 2007. Effect of Their Photoperiod Regimes The Growth and Mortality of the Japanese Abalone (Haliotis discus hanaino). Kochi University, Aquaculture Department, Laboratory of Fish Nutrition, Japan, 2(6): 763–767.
  81. Prajayati, VTF; Hasan, ODS; and Mulyono, M. 2020. Magot Flour PerformanceIncreases Formula Feed Efficiency and Growth of Nirwana Race Tilapia (Oreochromis sp.). Gadjah Mada University Fisheries Journal, 22(1): 27–35.
  82. Pratiwi, DY 2022. The Effect of Using Indigofera zollingeriana Leaf Meal as a fish feed ingredient. Aquatech Journal, 3(1): 27–32.
  83. Priadana, MS, and Sunarsi, D. 2021. Quantitative research methods. Pascal Books.
  84. Purbomartono, C., Pranannisa, SN, Mulia, DS, and Suwarsito, S. 2023. Growth of Patin Fish with the Addition of Ginger Flour Supplement Feed into the Biofloc System. JRST (Journal of Science and Technology Research), 7(1): 93-102.
  85. Puspita, EV, and RP Sari, 2018. Effect of Different Stocking Densities on Growth Rate of Catfish (Clarias gariepinus, Burch) Cultured in Biofloc and Nitrobacter Media. Aquascience. 6(2): 563-567.
  86. Putra, BP; Thaib, A.; and Nazlia, S. 2023. Effect of Using Leaf Flour Indigofera zollingeriana Fermentation Results of Lactobacillus sp. With Different Times in Feed Formula on the Growth of Tilapia (Oreochromis niloticus) Seeds. TILAPIA Journal: 4(1): 27–35.
  87. Putri, AJ, Lumbessy, SY, and Lestari, DP 2021. Substitution of Seaweed Flour Eucheuma striatum in Tilapia (Oreochromis niloticus) Fish Feed. Bioscientist: Biological Scientific Journal, 9(2), 333–345.
  88. Que F, Liu Z, Hu Y, Zhao Q, Zhou Y, Liu Z, Zhong L, Lu S, and Li J. 2019. Effects of dietary glutamine supplementation on growth performance and antioxidant status and intestinal function in juvenile grass carp (Ctenopharyngodon idella). Aquac Nutr, 25:609–621
  89. Rahmawati, AS, and Erina, R. 2020. Completely Randomized Design (CRD) with Test Two-way ANOVA. OPTICA: Journal of Physics Education, 4(1): 54–62.
  90. Reeds, PJ, DG Burnin, Stoll, and F. Jahoor. 2000. Glutamate and Glutamine in Metabolism: Intestinal Glutamate Metabolism. The Journal of Nutrition 130: 978S-982S.
  91. Rerung, RR Ed. 2023. Textbook of Agricultural Biochemistry. Media Sains Indonesia 59
  92. Restianti, A., Rachmawati, D., and Samidjan, I. 2016. Effect of Phytase Dosage in Artificial Feed on Growth and Efficiency of Feed Utilization for Larasati Tilapia (Oreochromis niloticus). The Journal of Aquaculture Management and Technology, 5(2): 35–43.
  93. Rochmah, DL, & Utami, ET 2022. The Impact of Consuming Monosodium Glutamate (MSG) on children's brain development. Journal of Public Health (Undip), 10(2): 163–166.
  94. Sampebatu, L., & Kamolan, A. (2021). Design and Development of Automation and Telecontrol Systems for Water Conditioning Tilapia Breeding Ponds, Jurnal Ampere, 6(1), 32–42.
  95. Sangadji, II 2022. Animal Husbandry Technology. Increasing the nutritional value of ruminant feed CV. Azka Pustaka.
  96. Sanutra, S., Syazali, M., and Erfan, M. 2022. Identification of Fish Types Found in the Ampenan River, Mataram, West Nusa Tenggara. BIOCHEPHY: Journal of Science Education, 2(2): 47–52.
  97. Salsabila, M., and Suprapto, H. (2018). Enlargement technique of tilapia (Oreochromis niloticus) in Freshwater Aquaculture Installation Pandaan, East Java. Journal of Aquaculture and Fish Health, 7(3): 118–123.
  98. Saputry, AM, and Latuconsina, H. 2022. Evaluation of Tilapia (Oreochromis niloticus) Fish Hatchery in Aquaculture Installations, Kepanjen-Regency Malang, JUSTE (Journal of Science and Technology), 3(1): 80–89.
  99. Sharma, V., Deshmukh, and R. Ajinomoto (MSG). A Fifth Taste or a Bio Bomb. EJPMR. 5(2): 381-400.
  100. Siegers, WH, Prayitno, Y., and Sari, A. 2019. The effect of water quality on the growth of Nirvana tilapia (Oreochromis sp.) in brackish ponds. The
  101. Journal of Fisheries Development, 3(2): 95–104.
  102. SNI.2018. TITLE. SNI 7242-2018. BSNI. Jakarta. 11 pp.
  103. SNI (Indonesian National Standards). 2009. Production of Tilapia (Oreochromis niloticus bleeker) Enlargement. In: Tranquil Water Pool, 12 pp.
  104. Solares, AC, I. Viegas, M. Salgado, AM Siles, A. Saez, I. Meton, Baanante, and F.Fernandez. 2015. Diets Supplemented with Glutamate or Glutamine Improve Protein Retention and Modulate Gene Expression of Key Enzymes of Hepatic Metabolismee in Glilthead Seabream (Spratus Aurata) Juveniles. Aquaculture.444(1): 79-87.
  105. Steffens, W. (1989). Principles of Fish Nutrition. Ellis Horwood Limited, West Sussex. England. 384 pp.
  106. Stickney, RR (1979). Principles of Warm Water Aquaculture. John Wiley and Sons, Inc.
  107. New York, pp. 223–229.
  108. Subandiyono and S. Hastuti. 2014. Baronang and the Prospects of Marine Cultivation in Indonesia, Ed. I., UPT Undip Press, Semarang, 79 pp.
  109. Suprayitno, E., Sulistiyati, TD, Panjaitan, MAP, Tambunan, JE, Djamaludin,H., and Islamy, RA 2021. Biochemistry of Fishery Products. Brawijaya University Press.60
  110. Surianti, SP, Damis, SK, Putri, ARS, Pi, S., and Hasrianti, SP 2022. Modification Tofu dries into raw material for shrimp and fish feed. Media Sains
  111. Indonesia.
  112. Suryanto, D., and Suprianto, B. 2021. The Effect of Feeding with Different Formulations for the Growth of Salin Tilapia (Oreochromis niloticus). Journal Airaha, 10(2): 248–254.
  113. Tacon, 1987. The Nutrition and Feeding of Farmer Fish and Shrimp: A. Training Manual, FAO of the United Nations, Brazil, 106–109.
  114. Tahapari, E., and N. Suhenda. 2009. Determining Feeding Frequency to Support the Growth of Pasupati Patin Fish Seeds. Biology News, 9(6): 693–693
  115. Takeuchi, T. 1988. Laboratory Work-Chemical Evaluation of Dietary Nutrients, in Watanabe, T., 1988. Fish Nutrition and Mariculture. Kanagawa International Fisheries Training Center, JICA Text Book, the General Aquaculture Course. Department of Aquatic Biosciences. Tokyo University of Fishseries, p. 233.
  116. Tinnagon, Tarrakoon, NC, 1999. The Nutritive Value of Banana Peel (Musa sapieutum L.) in Growing Oreochromis niloticus.
  117. Torii K., Uneyama H., and Nakamura E. (2013). Physiological roles of dietary glutamate signaling via the gut-brain axis due to efficient digestion and absorption. Journal of Gastroenterology, 4(8): 442-451.
  118. Trisnawati, Y., Suminto, and A. Sudaryono. 2014. The Effect of a Combination of Artificial Feed and earthworms (Lumbricus rubellus) on the efficiency of food utilization, growth, and survival of dumbo catfish (Clarias gariepinus). J.of Aquaculture Management and Technology, 3(2): 86–93.
  119. Wahyuni, RD, Yulinda, E., and Bathara, L. 2020. Break Even Point Analysis and Risks of Nile Tilapia (Oreochromis niloticus) Rearing Business in Keramba Floating Nets (KJA) in Pulau Terap Village, Kuok District, Kampar Regency Riau Province. Journal of Coastal Socioeconomics, 1(1): 22–33.
  120. Webster, SD, and Lim, C., 2002. Nutrient Requirements and Feeding of Finfish for Aquaculture Research Center, CABI Publishing, New York.
  121. Widiyati, A., Subandriyo, S., Sumantadinata, K., Hadie, W., and Nugroho, E. 2017. Morphological Diversity and Asymmetry Fluctuations of Tilapia (Oreochromis niloticus) from Lake Tempe (South Sulawesi) and several production centers in West Java. Indonesian Fisheries Research Journal, 10(5): 47–53.
  122. Wijayanti, M., Khotimah, H., Sasanti, AD, Dwinanti, SH, and Anggana, M. 2019.Maintaining Tilapia (Oreochromis niloticus) with System Aquaponics in Karang Endah Village, Gelumbang District, Muara Enim Regency South Sumatra. Journal Aquaculture and Fish Health, 8(3):139–148.
  123. Williams, MB, Dennis-Cornelius, LN, Miyasaki, ND, Barry, RJ, Powell, ML, Makowsky, RA, and Smith Jr., DL 2022. Macronutrient Ratio Modification in a Semi-Purified Diet Composition: Effects on Growth and Body Composition of juvenile zebrafish Danio rerio. North American Journal of Aquaculture,84(4): 493-504.
  124. Wilson, WA; Wang, Z.; and Roach, PJ, 2002. Systematic identification of the genes Affecting glycogen storage in the yeast Saccharomyces cerevisiae: implications of the vacuole as a determinant of glycogen level. Molecular cell proteomics,1(3), pp. 232-242.
  125. Wu, Guang. 2013. Amino Acids: Biochemistry and Nutrition. CRC Press, Boca Raton Yan L., Zhou XQ. (2006) Dietary glutamine supplementation improves structure and Function of the Intestine of the Juvenile Jian Carp Cyprinus carpio var. Jian.
  126. Aquaculture 25(6): 389–394.
  127. Yanto, H., RH Setawan, EI Raharjo, and Farida. 2018. The Effect of Fermented Rice Bran in Diets on Growth and Feed Efficiency of Jelawat (Leptobarbus hoevenii Blkr). Ruaya Journal 6, 30-37.
  128. Yonata, A., and I. Iswara, 2016. Toxic Effects of Consuming Monosodium Glutamate. Journal Majority. 5(3): 100–104.
  129. Yulintine, Y., Maryani, M., and Siburian, HS 2023. The Effect of Providing Different Types of Feed on the Survival and Growth of Snakehead Fish (Channa striata) Larvae. Journal of River and Lake Aquaculture, 8(1): 38–47.
  130. Zhao Y, Hu Y, Zhou XQ, Zeng XY, Feng L, Liu Y, and Jiang WD Li SH, Li DB, Wu XQ, Wu CM, Jiang J. 2015. Effect of Dietary Glutamate Supplementation on Growth Performance, Digestive Enzyme Activities, and Antioxidant Capacity in Intestine of Grass Carp Ctenopharyngodon idella. Aquaculture Nutrition.
  131. :935-941.
  132. Zhelyazkov. GI 2018. Effect of Monosodium Glutamate on Growth Performance and Blood Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss W). Agricultural Science and Technology, 10(3), 204–207.
  133. Zonneveld, N., EA Huisman, and JH Boon. 1991. Principles of Fish Cultivation. Gramedia Pustaka Utama, Jakarta, 318.