Article Sidebar

Submitted
Apr 21, 2024
Published
May 5, 2024
Download
Fulltext PDF
Statistic
Read Counter : 0 Download : 0

Main Article Content

Abstract

Giant gouramy is one of economical important freshwater fish. However, the production of the fish was declined because of a Motile Aeromonas Septicemia (MAS) disease. Giant gouramy with MAS disease caused an ulcer on their skin, and the worst case infection may cause death. The symptoms is vary widely depends on fish resistance to the disease. The aim of this study is to analyze microsatellite alleles that potential as a resistance marker against Aeromonas hydrophila. In previous studies, eleven microsatellite loci were isolated from giant gouramy genome. These loci were tested on DNA from resistant and susceptible giant gouramy that had been treated with Aeromonas hydrophila. Resistant giant gouramy was the gouramy that survived at least 50 days post-infection and ssusceptible giant gouramy was the gouramy that died before 50 days post- infection. Three of eleven microsatellite loci were found with unique alleles that appeared only in resistant giant gouramy and potential as resistance marker, which is the 342 bp allele at GE 1.9 locus with (GCA)10 (ACA) 6 motif, the 262 bp allele at GE 2.4 locus with (GCA) 6 (GGA)10 motif, and the 244 bp allele at GE 1.4 locus with (GCT) 9 (TA) 6 motif. All three loci were used to scan 48 giant gouramy broodstock from Tasikmalaya, Singaparna, and Sukabumi. Amplification of the microsatellite loci was performed by PCR with M13 tail sequence in forward primer and fluorescence dye. Successfully amplified alleles were analyzed with GeneAlEx 6.5 software. As a result, fragment 262 bp and 244 bp that contained in 43.75% of 48 gouramy broodstock predicted have a potency as resistance marker to Aeromonas hydrophila. For further study, microsatellite motifs have to be screened in gouramy progeny, because microsatellites are inhereted in Mendelian traits.

Keywords

Aeromonas hydrophila; giant gouramy; microsatellite

Article Details

License

Copyright (c) 2014 Aquacultura Indonesiana

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biography

Meirina Kartika Kusumawardhani, Bandung Institute of Technology

Department of Biotechnology, School of Life Science and Technology, 

References

  1. Chopra, A.K., X.J. Xu, D. Ribardo, M. Gonzalez, K. Kuhl, J.W. Peterson, and
  2. C.W. Houston. 2000. The cytotoxic enterotoxin of Aeromonas hydrophila induces proinflammatory cytokine production and activates arachidonic acid metabolism in macrophages. Infect. Immun., 68 : 2808–2818.
  3. Cota, Laura Cristina, P. Doru, B. Constantin, and G. Mircea. 2010. Preliminary Studies on Microsatellite Marker Analysis of Resistance to Common Bunt in several Wheat Genotypes (Triticum aestivum L.). Not. Bot. Hort. Agrobot. Cluj., 38 (2) : 42-47.
  4. Directorate General of Aquaculture. 2013. Statistik Menakar Target Ikan Air Tawar Tahun 2013. Written at : 2013-04-05.
  5. <http://www.djpb.kkp.go.id/berita.php?id
  6. =847>.
  7. Gosling, P.J. 1996. Pathogenic mechanisms in The genus Aeromonas, eds Austin B. et al., Wiley, London, pp. 245–265.
  8. Howard, S.P., S. MacIntyre, and J.T. Buckley. 1996. Toxins in: The genus Aeromonas, eds Austin B. et al., Wiley, London, pp. 267–286.
  9. Kashi, Y. and M. Soller. 1999. “Functional Roles of Microsatellites and Minisatellites in Microsatellites: Evolution and Applications, eds Goldstein and Schlotterer, Oxford University Press. Oxford.
  10. Kusumawaty, D., M.M. Margrita, N.R. Arma, and A. Pancoro. 2005a. Isolation and Characterization MicrosatellitesLocus in
  11. Osphronemus gouramy. International Procceding seminars: ASEAN Sience and Technology week (7th ASTW): Biotechnology for suistainable utilization of biological resources in agriculture and health in ASEAN.
  12. Kusumawaty, D, M.M. Margrita, D. Rochintaniawati, F.S. Kurniadie, M.R. Moeis, dan A. Pancoro. 2005b. Seleksi ikan gurame varietas blusafir yang resisten terhadap infeksi bakteri Aeromonas hydrophila klon 26. Prosesing Seminar Nasional dan Kongres Biologi XIII, Yogyakarta.
  13. Kwok, S., D.E. Kellog, N. McKinney, D. Spasic, L. Goda, and C. Levenson. 1990. Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus 1 model studies. Nucleic Acids Res., 18 : 999–1005.
  14. Noga, E.J. 2011. Fish disease : Diagnosis and treatment. second edition. John Wiley and Sons,. USA.
  15. Pokhriyal, B., K. Thorat, D.A. Limaye, Y.M. Joshi, V.J. Kadam, and R. Dubey. 2012. Microsatellite Markers – A Novel Tool In Molecular Genetics, International Journal Of Research In Pharmacy and Chemistry, 2 (2).
  16. Swann, L.D. and M.R. White. 1991. Diagnosis and Treatment of “Aeromonas hydrophila” Infection of Fish, Aquaculture Extension, Sea Grant # IL- IN-SG-FS-91-2 Fact Sheet AS-461,
  17. Illinois-Indiana Sea Grant Program, Purdue University.
  18. Toonen, R.J. 1997. Microsatellites for Ecologists: Non-radioactive isolation and amplification protocols for microsatellite markers. Available in
  19. <http://www2.hawaii.edu/∼toonen/files/ Msats-for- Ecologists-V1.pdf.>. [April 24, 2014].
  20. Uribe, C., H. Folch, R. Enriquez, and G. Moran. 2011. Innate and adaptive immunity in teleost fish: a review, Veterinarni Medicina, 56 (10): 486–503.