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Apr 23, 2024
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May 5, 2024
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Abstract

The spermatozoa quality of goramy after 2 d sub-zero freezing was examined. The quality of spermatozoa examined included motility, viability, and abnormality. We aimed to determine the optimum concentration of glycerol protecting spermatozoa during preservation. We used 0%, 1%, 3%, 5%, 7%, and 9% of glycerol, respectively. Sperms were diluted by the combination of glycerol and fish ringer (1 part of sperm + 3 part of solvent). The dilute sperms were then equiliberated at 4°C for 45 min, and were freezed at -34°C for 2 d. Thawing was then carried out at 30°C for 2 min. Based on Dunnet test, 5% of glycerol was the optimum concentration maintaining spermatozoa motility (75.95±4.76)%.

Keywords

Glycerol; Osphronemus goramy; Spermatozoa motility; Sub-zero freezing; Viability and Abnormality

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Copyright (c) 2015 Aquacultura Indonesiana

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biographies

Abinawanto Abinawanto, Universitas Indonesia, Depok, Indonesia

Biology Graduate Study Program, Faculty of Mathematics and Natural Sciences

Nisa Fitrianingrum, Universitas Indonesia, Depok, Indonesia

Biology Graduate Study Program, Faculty of Mathematics and Natural Sciences

Retno Lestari, Universitas Indonesia, Depok, Indonesia

Biology Graduate Study Program, Faculty of Mathematics and Natural Sciences

Agung Sudaryono, Diponegoro University, Semarang, Indonesia

Aquaculture Study Program

Rita Rostika, Padjadjaran University, Bandung, Indonesia

Faculty of Fisheries and Marine Science

Yushinta Fujaya, Hasanudin University, Makassar, Indonesia

Faculty od Marine Science and Fisheries

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