Main Article Content
Abstract
In this study, the effects of oral administration of probiotic Bacillus sp. D2.2 and prebiotic from sweet potato extract on growth performance and resistance against Vibrio harveyi in Pacific white shrimp (Litopenaeus vannamei) were investigated. During 32-day feeding experiment, 360 individuals of Pacific white shrimp (PL15) with initial weight of 0.02 ± 0.002 g were fed with basal diet as control (A); supplemented with 6% probiotic and 0% prebiotic (B); 6% probiotic and 2% prebiotic (C); 6% probiotic and 4% prebiotic (D). At the end of feeding trial, weight gain (WG), average daily growth (ADG), feed conversion ratio (FCR), and survival rate (SR) were assessed. WG, ADG and FCR of the shrimp were significantly better in treatment D than those of the shrimp in other treatments. Control and treatment D as the best feeding trial were selected for challenge test with infectious V. harveyi. Survival rate and mean time to death (MTD) of the shrimp fed the supplemented diet were not significantly different (P>0.05) to the control. Infection levels in shrimp were evaluated using morphological scoring methods. Infection levels of V. harveyi in shrimp fed the diet were lower compared to the control.
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Copyright (c) 2017 Aquacultura Indonesiana

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References
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- immune responses and resistance to white spot
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- vannamei). Aquaculture. 291: 35-40.
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- Dietary supplementation of short-chain fructooligosaccharides influences gastrointestinal
- microbiota composition and immunity
- characteristics of Pacific white shrimp,
- Litopenaeus vannamei, cultured in a
- recirculating system. Journal of Nutrition,
- : 2763-2768.
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- probiotic strain S12 and its effect on growth
- performance, non-specific immunity and
- disease-resistance of white shrimp, Litopenaeus
- vannamei. Fish & shellfish immunology, 41(2):
- -307.
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- Journal of Fisheries and Aquatic Science,
- (1): 13-23.
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- vannamei. HAYATI Journal of Biosciences, 21(3):
- -134.
- Partida-Aranguere, B.O., A. Luna-Gonzalez, J.A.
- Fierro-Coronado, M. del-Carmen Flores-
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- Effect of inulin and probiotic bacteria on
- growth, survival, immune response, and
- prevalence of white spot syndrome virus
- (WSSV) in Litopenaeus vannamei cultured
- under laboratory conditions. Afr. J.
- Biotechnol., 12: 3366–3375.
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- : 301–313.
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- Immunity enhancement in black tiger
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- (Penaeus monodon) di Lampung Timur strain
- D.2.2, terhadap bakteri patogen pada udang
- dan ikan. Prosiding Pertemuan Ahli
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- A.B.P. Medeiros, C.T. Yamaguishi, J.D.D.
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- review. Food Technology and Biotechnology,
- (4):413-434.
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- Efektivitas sinbiotik dengan dosis berbeda
- pada pemeliharaan udang vaname di tambak.
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- doi:10.1111/j.1365-2109.2011.02936.x U.S. Food and Drug Administration. 2008. Fish and Fishery Products Hazards and Controls Guidance Department of Health and Human Services, Food and Drug Administration, Center for Food Safety and Applied Nutrition Medicine. http://www.fda.gov/downloads/food/guidanceregulation/ucm251970.pdf.
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- environment: a link to the clinic. Current Opinion in Microbiology, 13(5): 589-594.
- Xiong, J., K. Wang, J. Wu, L. Qiuqian, K. Yang, Y.
- Qian, and D. Zhang. 2015. Changes in intestinal bacterial communities are closely associated with shrimp disease severity. Applied microbiology and biotechnology,99(16): 6911-6919.
- Zhou Z., Z. Ding and H.Y. Lv. 2007. Effects of dietary short-chain fructooligosaccharides on intestinal microflora, survival and growth performance of juvenile Pacific white shrimp Litopenaeus vannamei. Journal of the World Aquaculture Society, 38: 296-301.
- Zokaeifar, H., Balcázar, J. L., Saad, C. R., Kamarudin, M. S., Sijam, K., Arshad, A., and Nejat, N. 2012. Effects of Bacillus subtilis on the growth performance, digestive enzymes, immune gene expression and disease resistance of white shrimp, Litopenaeus vannamei. Fish & shellfish immunology, 33(4): 683-689.
References
Adel, M., S. Yeganeh, M.A.O. Dawood, R. Safari,
and S. Radhakrishnan. 2017. Effects of
Pediococcus pentosaceus supplementation on
growth performance, intestinal microflora and
disease resistance of white shrimp, Litopenaeus
vannamei. Aquaculture Nutrition, 23(6): 1401-
Ai, Q., H. Xu, K. Mai, W. Xu, J. Wang, and W.
Zhang. 2011. Effects of dietary
supplementation of Bacillus subtilis and
fructooligosaccharide on growth performance,
survival, non-specific immune response and
disease resistance of juvenile large yellow
croaker, Larimichthys crocea, Aquaculture,
:155-161.
Arisa, I.I. 2011. Application of probiotic, prebiotic
and synbiotic to enhance the immune
response of shrimp Litopenaeus vannamei
against Vibrio harveyi infection. Master
Thesis, Bogor Agricultural University,
Indonesia.
Azhar, F. 2013. The effects of probiotic and prebiotic
on performance of juvenile polkadot grouper
Cromileptes alvelis. Buletin Veteriner
Udayana, 6: 1-9.
Bell, T. A. and D.V. Lightner. 1988. A handbook of
normal penaeid shrimp histology (No. 595.38
B4). Word Aquaculture Society, 114p.
Bengmark, S. 2005. Bioecologic control of the
gastrointestinal tract: the role of flora and
supplemented probiotics and synbiotics.
Gastroenterology Clinics of North America,
:413-436.
Cerezuela, R., F.A. Guardiola, J. Meseguer, and
M.A. Esteban. 2012. Increases in immune
parameters by inulin and Bacillus subtilis
dietary administration to gilthead seabream
(Sparus aurata L.) did not correlate with
disease resistance to Photobacterium damselae.
Fish Shellfish Immunol., 32:1032-1040.
Daniels, C.L., D. L. Merrifield, D.P. Boothroyd,
S.J. Davies, J.R. Factor, and K.E. Arnold.
Effect of dietary Bacillus spp. and
mannan oligosaccharides (MOS) onEuropean
Lobster (Homarus gammarus L.) larvae
growth performance, gut morphology and gut
microbiota. Aquaculture, 304:49-57.
Dimitroglou, A., D.L Merrifield, R. Moate, S.J.
Davies, P. Spring, J. Sweetman, and G.
Bradley. 2009. Dietary mannan oligosaccharide
supplementation modulates intestinal microbial
ecology and improves gut morphology of
rainbow trout, Oncorhynchus mykiss
(Walbaum). Journal of animal science, 87(10):
-3234.
Gibson, G., H. Probert, J. Loo, R. Rastall, and M.
Roberfroid. 2004. Dietary modulation of the
human colonic microbiota: Updating the
concept of prebiotics. Nutrition Research
Reviews, 17(2):259-275. doi:10.1079/NRR200479.
Gibson G.R. and M.B. Roberfroid. 1995. Dietary
modulation of the human colonic microbiota:
introducing the concept of prebiotics. Journal
of Nutrition, 125: 1401-1412.
Hai, N.V. and R. Fotedar. 2009. Comparison of the
effects of prebiotics (Bio-Mos® and ß-1, 3-
D-glucan) and the customized probiotics
(Pseudomonas synxantha and P. aeruginosa)
on the culture of juvenile western king prawn
(Penaeus latisulcatus Kishinouye, 1896).
Aquaculture, 289:310-316.
Hardiyani, S., E. Harpeni, A. Setyawan and
Supono. 2016. Pathogebicity and in vivo
study of local isolate Bacillus sp. D2.2 at the
vannamei culture (Litopenaeus vannamei).
Aquasains, 5(1): 421-425.
Harpeni, E., A. Setyawan, L. Santoso and Z. Arifin.
Efektivitas ekstrak tepung ubi jalar
sebagai media teknis bakteri probiotik.
Proceeding Seminar Nasional MIPA 2016:
- 130. Jatinangor, 27-28 Oktober 2016.
Joshi, J., J. Srisala, V.H. Truong, I.T. Chen, B.
Nuangsaeng, O. Suthienkul, C.F. Lo, T.W.
Flegel, K. Sritunyalucksana, and S.
Thitamadee. 2014. Variation in Vibrio
parahaemolyticus isolates from a single Thai
shrimp farm experiencing an outbreak of
acute hepatopancreatic necrosis disease
(AHPND). Aquaculture, 428: 297-302.
Khimmakthong, U., P. Deachamag, A. Phongdara,
and W. Chotigeat. 2011. Stimulating the
immune response of using the phagocytosis
activating protein (PAP) gene, Fish &
Shellfish Immunology, 31(3): 415-422.
Li J.Q., B.P. Tan and K.S. Mai. 2009. Dietary probiotic
Bacillus OJ and isomaltooligo saccharides
influence the intestine microbial populations,
immune responses and resistance to white spot
syndrome virus in shrimp (Litopenaeus
vannamei). Aquaculture. 291: 35-40.
Li P., G.S. Burr, D.M III. Gatlin, M.E. Hume, S.
Patnaik, F.L. Castille, and A.L. Lawrence.
Dietary supplementation of short-chain fructooligosaccharides influences gastrointestinal
microbiota composition and immunity
characteristics of Pacific white shrimp,
Litopenaeus vannamei, cultured in a
recirculating system. Journal of Nutrition,
: 2763-2768.
Liu, H., Z. Li, B. Tan, Y. Lao, Z. Duan, W. Sun,
and X. Dong. 2014. Isolation of a putative
probiotic strain S12 and its effect on growth
performance, non-specific immunity and
disease-resistance of white shrimp, Litopenaeus
vannamei. Fish & shellfish immunology, 41(2):
-307.
Nitimulyo, K.H., A. Isnansetyo, Triyanto, M.
Murdjani, and L. Sholichah. 2005.
Efektivitas vaksin polivalen untuk pengendalian
vibriosis pada kerapu tikus (Cromileptes
altivelis). J. Fish. Sci., 7(2): 95-100.
Nurhayati, D., Widanarni, and M. Yuhana. 2015.
Dietary symbiotic influence on growth
performances and immune responses to co-
infection with infectious myonecrosis virus
and Vibrio harveyi in Litopenaeus vannamei.
Journal of Fisheries and Aquatic Science,
(1): 13-23.
Oktaviana, A., Widanarni and Yuhana, M. 2014.
The Use of Synbiotics to Prevent IMNV and
Vibrio harveyi Co-Infection in Litopenaeus
vannamei. HAYATI Journal of Biosciences, 21(3):
-134.
Partida-Aranguere, B.O., A. Luna-Gonzalez, J.A.
Fierro-Coronado, M. del-Carmen Flores-
Miranda, and H.A. Gonzalez-Ocampo. 2013.
Effect of inulin and probiotic bacteria on
growth, survival, immune response, and
prevalence of white spot syndrome virus
(WSSV) in Litopenaeus vannamei cultured
under laboratory conditions. Afr. J.
Biotechnol., 12: 3366–3375.
Rengpipat, S., W. Phianphak, S. Piyatiratitivorakul,
and P. Menasveta. 1998. Effects of a probiotic
bacterium on black tiger shrimp (Penaeus
vannamei) survival and growth. Aquaculture,
: 301–313.
Rengpipat, S., S. Rukpratanporn, S.
Piyatiratitivorakul, and P. Menasveta.
Immunity enhancement in black tiger
shrimp (Penaeus vannamei) by a probiont
bacterium Bacillus S11. Aquaculture, 191:
–288.
Rengpipat, S., A. Tunyamum, A.W. Fast, S.
Piyatiratitivoraku, and P. Menasveta.
Enhanced growth and resistance to
vibrio challenge in pond-reared black tiger
shrimp Penaeus monodon fed a Bacillus
probiotic. Diseases of Aquatic Organisms 55:
-173.
Ringo, E., R.E. Olsen, T.O. Gifstad, R.A. Dalmo,
H. Amlund, G.I. Hemre, and A.M. Bakke.
Prebiotics in aquaculture: A review.
Aquacult. Nutr., 16: 117-136.
Setyawan, A., E. Harpeni, M. Ali, D. Mariska, and
M. Aji. 2014. Potensi agen bakteri biokontrol
indigenous tambak tradisional udang windu
(Penaeus monodon) di Lampung Timur strain
D.2.2, terhadap bakteri patogen pada udang
dan ikan. Prosiding Pertemuan Ahli
Kesehatan Ikan 2014. Serang 11-13 Februari
pp. 24-31.
Soccol, C.R., L.P.d.S. Vandenberghe, M.R. Spier,
A.B.P. Medeiros, C.T. Yamaguishi, J.D.D.
Lindner, A. Pandey, and V. Thomaz-Soccol.
The potential of probiotics: a
review. Food Technology and Biotechnology,
(4):413-434.
Sukenda, R. Praseto, and Widanarni. 2015.
Efektivitas sinbiotik dengan dosis berbeda
pada pemeliharaan udang vaname di tambak.
Jurnal Akuakultur Indonesia,14(1): 1-8.
Sun, Y., Wen, Z., Li, X., Meng, N., Mi, R., Li, Y.and Li, S. 2012. Dietary supplement of fructooligosaccharides and Bacillus subtilis enhances the growth rate and disease resistance of the sea cucumber Apostichopus japonicus (Selenka). Aquac. Res., 43:1328–
doi:10.1111/j.1365-2109.2011.02936.x U.S. Food and Drug Administration. 2008. Fish and Fishery Products Hazards and Controls Guidance Department of Health and Human Services, Food and Drug Administration, Center for Food Safety and Applied Nutrition Medicine. http://www.fda.gov/downloads/food/guidanceregulation/ucm251970.pdf.
Verschuere, L., G. Rombaut, P. Sorgeloos, and W.
Verstraete. 2000. Probiotic Bacteria as Biological Control Agents in Aquaculture. Microbiology and Molecular Biology, 64(4):655-671.
Wright, G. D. 2010. Antibiotic resistance in the
environment: a link to the clinic. Current Opinion in Microbiology, 13(5): 589-594.
Xiong, J., K. Wang, J. Wu, L. Qiuqian, K. Yang, Y.
Qian, and D. Zhang. 2015. Changes in intestinal bacterial communities are closely associated with shrimp disease severity. Applied microbiology and biotechnology,99(16): 6911-6919.
Zhou Z., Z. Ding and H.Y. Lv. 2007. Effects of dietary short-chain fructooligosaccharides on intestinal microflora, survival and growth performance of juvenile Pacific white shrimp Litopenaeus vannamei. Journal of the World Aquaculture Society, 38: 296-301.
Zokaeifar, H., Balcázar, J. L., Saad, C. R., Kamarudin, M. S., Sijam, K., Arshad, A., and Nejat, N. 2012. Effects of Bacillus subtilis on the growth performance, digestive enzymes, immune gene expression and disease resistance of white shrimp, Litopenaeus vannamei. Fish & shellfish immunology, 33(4): 683-689.
