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Abstract
This research was conducted in Institute for Mariculture Research and Development
(IMRAD) using on concrete tanks 150 m3. The objective of this research was to know the performance and gonad maturations development and spawn activities of first generation (G-1) broodstock from individual selection. The experiment test of milkfish broodstock with weight size of 3,891.67±344.99 g were reared on tank. Fish were reared at initial density of 60 individuals fish in 150 m3 concrete tanks. The treatments were: with hormone (A) and without hormone (B) implantation. The doze of LHRH-a implant hormone was 50 μg/kg. The broodstock were reared using of standard procedure operational (SOP) for spawning and gonad maturations. The result of the experiment showed that fish with hormone implantation gave the better results in gonad development on stage of reproduction than fish without hormon implantation. The gonad maturations and development on male and female broodstock diameter of oocyte and sperm were 450 μm and positive 2 and 3, respectively. The broodstock was spawn on the continue rear culture in the tank. The frequency of spawning were 8 times (with hormone) and 4 times (without hormone implantation) with total number of egg was 1,660,000 and 500,000 pc, respectively. The quality of eggs were 55-95% for fertilities and index activity survival (SAI) after hatching larvae was 4-5 days.
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References
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References
Chen, L.C. and R.L. Martinich. 1975. Pheromonal stimulations and metabolite inhibitions of ovulations in zebrafish, Brachydanto rerio. In: W.S. Hoar and D.J. Randall (Eds.), Fish Physiology. Academic Press, New York. IX(B): 1-63.
Djawad, I.M. 1997. Studies on the metabolism of rearing fish larvae. Doctor of Philosophy. Disertations. Hirosima University.
Effendi. 1979. Metoda Biologi Perikanan. Yayasan Dewi Sri. Bogor. 112 pp.
Effendi, H. 2003. Telaah Kualitas Air Bagi Pengelolaan Sumber Daya dan Lingkungan Perairan. Penerbit Kanisius Jogyakarta.258 p.
Lee, C.S., C.S. Tamaru, and C.D. Kelly. 1986. Technique making chronic release LHRH-a and 17-alpha methyl testosteron pellet for intramusculer implantation in fishes. Aquaculture, 59:161-168.
Mayunar, P.T. Imanto, S. Diani dan T. Yokonawa.
Pemijahan Ikan Kerapu Macan, Epinephelus fuscoguttatus. Bull.Pen. Perikanan (Terbitan khusus), 2:15-22.
Priyono. A, T. Ahmad, dan T. Setiadharma. 1993. Pengaruh penambahan nutrisi pakan terhadap perkembangan gonad ikan bandeng. J. Pen. Budidaya Pantai, 9(1): 51-57.
Priyono, A., T. Setiadharma, P.T. Imanto, M. Swastika, dan Z.I. Azwar. 2004. Pengaruh dosis pelet hormone terhadap perkembangan sel telur dan gonad induk betina kakap merah, Lutjanus argentimaculatus. Prosiding Lokakarya Perhimpunan Ilmu Pemuliaan Indonesia, VII:575-582.
Priyono, A., T. Aslianti, T. Setiadharma, dan I.N.
Adiasmara Giri. 2011. Petunjuk Teknis Perbenihan Ikan Bandeng (Chanos chanos Forskal). Balai Besar Penelitian dan Pengembangan Budidaya Laut. 44 pp.
Suwirya, K. 1994. Kecernaan beberapa sumber lemak
pakan pada udang windu, Penaeus monodon. Balai Penelitian Perikanan Budidaya Pantai. Maros. J. Penelitian Budidaya Pantai, 10(1): 43– 48.
Setiadharma T., A. Priyono, N.A. Giri, dan Wardoyo.
Aplikasi Penambahan Vitamin E dan C untuk Pematangan Gonad dan Menigkatkan Pemijahan serta kualitas telur Induk Kerapu Macan (Epinephelus fuscoguttatus). 8 Hlm. Inpress.
Tamaru, C.S, C.S. Lee, C.D. Kelly, J.E. Banno, P.Y. Ha, K. Aida, and I. Hanyu. 1987. Characterizing the stage of maturity most receptive to an acute LHRH-a therapy for inducing milkfish (Chanos-chanos) to spawn. Aquaculture, 74:147-163.
Tridjoko, S. Ismi, A. Prijono, dan F. Johnny. 2002.
Pengamatan profil steroid hormone dalam darah hubungannya dengan pematangan dan pemijahan induk ikan kerapu bebek, Cromileptes altivelis. Laporan penyelesaian DIP 2002, halaman. 40-54.
Watanabe,W.O, C.E. Simon, P.E. Eileen, O.H. William, D.K. Christopher, M. Aaron, S.L. Cheng, and K.B. Paul. 1995. Progress in controlled breeding of Nassau grouper, Epinephelus striatus broodstock by hormon induction. Aquaculture, 138:205-219.
