Article Sidebar

Submitted
Feb 11, 2026
Accepted
May 23, 2026
Published
May 29, 2026
Download
Fulltext Pdf
Statistic
Read Counter : 0 Download : 0

Main Article Content

Abstract

Giant freshwater prawn (Macrobrachium rosenbergii) is one of the freshwater commodities in Indonesia with high market demand. In order to increase the growth rate of these prawns, their feed is supplemented with papain enzyme and calcium. The former is a proteolytic enzyme that plays a role in breaking down proteins into simpler compounds of amino acids, allowing it to be more easily absorbed into the prawn’s body. The latter, i.e., calcium, plays a role in the molting process by accelerating the gastrolization process, which eventually can accelerate the growth rate. This study aims to test the effect of providing papain enzyme and calcium from crab shell flour (TCR) to the reprocessed commercial feed. The subjects tested in this study were 6-to-9-gram giant freshwater prawns about 9-cm long, at a density of 1 prawn/2 liters of water. The Completely Randomized Design (CRD) was used with 5 treatments and repeated 3 times. The treatments were referred to as P0 (Papain 0%; TCR 0%), P1 (Papain 2.25%; TCR 3%), P2 (Papain 3.375%; TCR 3%), P3 (Papain 5%; TCR 3%), and P4 (Papain 6%; TCR 3%). The best treatment was P3 (Papain 5%; TCR 3%) that had the values of EPP, FCR, PER, absolute weight growth, absolute length growth, RGR, SR, and molting frequency of 52.44%, 1.91, 9.99%, 3.74g, 2.08cm, 0.78%/day, 91%, and 1.38 times/head, respectively.

Keywords

Macrobrachium rosenbergii growth papain enzyme crab shell flour

Article Details

License

Copyright (c) 2026 Aquacultura Indonesiana

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

References

  1. Bahri, S., Mardhia, D., dan Saputra, O., 2022. Growth and Graduation of Vannamei Shell Life (Litopenaeus Vannamei) with Feeding Tray (ANCO) System in AV 8 Lim Shrimp Organization (LSO)IN Sumbawa District. Jurnal Biologi Tropis, 20(2): 279-289.
  2. Effendie, M.I., 1979. Metode Biologi Perikanan. Yayasan Dewi Sri, Bogor.
  3. Elsson, M., Wijanarko, A., Hermansyah, H., dan Sahlan, M., 2019. Michaelis-Menten Parameters Characterization of Commercial Papain Enzyme “Paya”. IOP Conference Series: Earth and Enviromental Science, 217: 012037.
  4. Handayani, L., dan Syahputra, F., 2018. Perbandingan Frekuensi Molting Lobster Air Tawar (Cherax quadricarinatus) yang Diberi Pakan Komersil dan Nanokalsium yang Berasal dari Cangkang Tiram (Crassostrea gigas). Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan, 7(1): 42-46.
  5. Hoseinifar, S. H., Faheem, M., Liaqat, I., Doan, H. V., Ghosh, K., dan Ringo, E., 2024. Promising Probiotic Candidates for Sustainable Aquaculture: An Updated Review. Animals, 14(24), 3644.
  6. Kurnia, A. Muskita, W. H., Astuti, O., Asnani dan Harahap, W., 2016. Evaluasi Penggunaan Tepung Cangkang Rajungan sebagai Bahan Baku Pakan Juwana Udang Windu Panaeus monodon. Jurnal Akuakultur Indonesia, 15(2): 117-123.
  7. Mahary, A., Wibowo, A. P., Sikta, A., Vygoc dan Handayani, R., 2022. Pemanfaatan Limbah Rajungan sebagai Pakan Ikan Lele di Desa Dahari Selebar Kabupaten Batubara. Prosiding Seminar Nasional Multidisiplin Ilmu Universitas Asahan, 464-471 hal.
  8. Mareta, R. E., Subandiyono dan Hastuti, S., 2017. Pengaruh Enzim Papain dan Probiotik dalam Pakan Terhadap Tingkat Efisiensi Pemanfaatan Pakan dan Pertumbuhan Ikan Gurami (Osphronemus gouramy). Jurnal Sains Akuakultur Tropis, 1:21-30.
  9. Patil, D.W., and Singh, H., 2014. Effect of Papain Suplemented Diet on Growth and Survival of Post-Larvae of Macrobrachium rosenbergii. International Journal of Fisheries and Aquatic Studies, 1(6).
  10. Prihatini, I., dan Dewi, R. K., 2021. Kandungan Enzim Papain pada Pepaya (Carica papaya L) Terhadap Metabolisme Tubuh. Jurnal Tadris IPA Indonesia, 1(3): 449-558.
  11. Puspasari, T., Y. Andriani, H. Hamdani, 2015. Pemanfaatan Bungkil Kacang Tanah Dalam Pakan Ikan Terhadap Laju Pertumbuhan Ikan Nila (Oreochromis niloticusi). Jurnal Perikanan Kelautan.06(02): 91-100 hlm
  12. Rachmawati, D., Hutabarat, J., Dewi, E. N., dan Windarto, S., 2020. Suplementasi Enzim Papain dalam Pakan Terhadap Performa Pertumbuhan, Efisiensi Pemanfaatan Pakan dan Kelulushidupan Udang Vanamei (Litopenaeus vannamei). Journal of Marine Research, 9(3): 215-222.
  13. Robi, Yasin, M. N., dan Yulintine, 2020. Penggunaan Enzim Papain pada Pakan Benih Ikan Gabus (Channa striata). Journal of Tropical Fisheries, 15(2): 78-86.
  14. Rostika, R., Pratiwy, F. M., dan Permana, R., 2021. The Effect of Papain Enzyme in Various Doses to The Growth and Intestinal of Cantang Grouper (Epinephelus fuscoguttatus-lanceolatus). IOP Conference Series: Earth and Enviromental Science, 763(1): 012022p.
  15. Rostika, R., Sunarto, H. N. Sugiyanto dan Dewanti, L. P., 2018. The Effectiveness of Crude Papain Enzyme Supplement for Tilapia’s (Oreochromis niloticus) Growth at The Floating Nets of Cirata Reservoir. IOP Canf. Series: Earth and Enviromental Science 139.
  16. Samadan, G. M., Muchdar, F., Sriwati dan Findra, M. N., 2023. Pengaruh Pemberian Kombinasi Pakan dan Probiotik yang Berbeda terhadap Kinerja Pertumbuhan Udang Galah (Macrobrachium rosenbergii) dan Penurunan Limbah N yang Dipelihara dalam Wadah Terkontrol. Juvenil: Jurnal Ilmiah Kelautan dan Perikanan, 6(2): 75-83.
  17. Suriyani, 2010. Karakteristik Kualitas Perairan Situ IPB, Kampus IPB Dramaga, Bogor. Skripsi. Departemen Manajemen Sumberdaya Perairan. Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor.
  18. Tacon, A.G., 1987. The Nutrition and Feeding of Farmed Fish and Shrimp. A Traning Mannual. FAO of The United Nations, Brazil, 106-109 p.
  19. Takeuchi, T., 1988. Laboratory Work-Chemical Evaluation of Dietary Nutrients. In: Watanabe, T. (Ed.). Fish Nutrition and Mariculture. JICA, Tokyo University Fish, 179-229 p.
  20. Tarigan, P. S. N., 2022. Pengaruh Pencampuran Tepung Cangkang Rajungan (Portunus pelagicus) pada Pakan Komersil terhadap Pertumbuhan dan Komposisi Karkas Ikan Nila (Oreochromis niloticus). Skripsi. Universitas Sumatera Utara.
  21. Wahyu, F., dan Chadijah, A., 2017. Penambahan Cangkang Rajungan pada Pakan untuk Intensitas Warna Ikan Mas Koi Kohaku. Jurnal Ilmiah Perikanan dan Kelautan, 5(3): 84-89.
  22. Zonneveld, N., Huisman, E. A., dan Boon, J. H., 1991. Prinsip-Prinsip Budidaya Ikan. PT. Gramedia Pustaka Utama, Jakarta, 318 hlm.
  23. Zufadhillah, S., Thaib, A., dan Handayani, L., 2018. Efektivitas Penambahan Nano CaO cangkang Kepiting Bakau (Syclla serrate) Kedalam Pakan Komersial terhadap Pertumbuhan dan Frekuensi Molting Udang Galah (Macrobrachium rosenbergii). Acta Aquatica: Aquatic Sciences Journal, 5(3).