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Apr 19, 2024
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May 5, 2024
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Abstract

Koi herpesvirus (KHV) is one of pathogen that infects common carp. Alternative to prevent KHV infection is trough vaccination This study aimed to observe gonad development and blood profile on the common carp after being vaccinated by GP25 plasmid as DNA anti-KHV vaccine. Vaccination was performed on common carp broodstock at 30, 45 and 60 days before spawning. Three fishes were used for each treatment. Three fishes were injected with phosphate buffer saline as control. Blood collection for profile analysis was taken at 15 days pre-vaccination and 30, 45, 60 days post-vaccination. Egg diameter was observed during vaccination and, when spawning was performed. Gonad somatic index was measured in spawning time. The results showed that gonad somatic index in vaccinated broodstock was 13.75±1.57% and broodstock control was 12.15±0.65%. Egg diameter were 0.57±0.06 – 0.97±013 mm at vaccination and 1.47±0.23 – 1.53±0.12 mm at spawning. There were differences morphological characteristic in fish at two different times. At vaccination time were abdomen still hard and genital papilla covered. While spawning time were abdomen appears rounded, enlarge and soft, papilla genital expands, widened anal and protruding Erythrocyte decreased significantly on 30 days post-vaccination ie 1.05±0.34 (x106 cells /mm3). Hemoglobin decreased on the 45 days ie 5.84±0.72 g/dL and hematocrite decreased on 30 days ie 41.48±10.90% post-vaccination. This study showed that anti-KHV GP25 DNA vaccine could reduce the erythrocytes, hemoglobin and hematocrite in normal level but not affecting in gonad development of common carp.

Keywords

Common carp; DNA vaccine; Gonad development; Koi herpesvirus

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Copyright (c) 2020 Aquacultura Indonesiana

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Author Biographies

Ardana Kurniaji, Polytechnic of Marine and Fisheries Bone, Bone, Indonesia

Study Program of Aquaculture Technique

Sri Nuryati, Bogor Agricultural University, Bogor, Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Science

Alimuddin Alimuddin, Bogor Agricultural University, Bogor, Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Science

Sri Murtini, Bogor Agricultural University, Bogor, Indonesia

Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine

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