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Abstract
Probiotics is a bacterial product that has the advantage for fish; it is not only for growing but also for covering the bacteria’s attack. The aim of the study is to determine the types of candidate bacteria’s probiotic of which the digestive tract of giant prawns in Teratak Buluh Pekanbaru, Riau. This research was conducted at the Laboratory of Microbiology and Biotechnology of Fisheries and Marine Sciences faculty, University of Riau, Pekanbaru to get a candidate of Probiotic Bacteria and conduct molecular examination that consists of purification of electrophoresis gel and 16S rDNA sequencing at the Institute for Biotechnology Serpong, Tangerang Banten. The method used in this researh was physical, biochemical and molecular test in order to identify the candidate bacteria. The results showed that the are eleven isolates of bacterial are the species Bacillus BDU4 strain from A Bacteria isolates with 99% homology and 10 isolates of bacterial are bacillus genus because the degree of homology below 97% and they are considered as the original indonesia’s new species probiotic bacteria.
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References
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- Sarkono, Faturrahman, and Y. Sofyan. 2010. Isolation and identification of lactic acid bacteria from parent abalone (Haliotis asinina) as a potential probiotic candidates. Biotechnology, 7 : 99-106.
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- Protease from Bacillus subtilis 1012M15. University of March, Surakarta.
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References
ABIS encyclopedia. 2009. Bacillus cereus http://www.tgw1916.net/ABIS/ encyclopedia.html. accessed : February 20, 2010.
Anggriani, R., Iskandar, and A. Taofiqurohman. 2012. Effectiveness addition of Bacillus sp. Results Isolation of Gastrointestinal Feeding Catfish in Commercial Against Seed Survival and Growth of Red Tilapia (Oreochromis
niloticus). Journal of Fisheries and Maritime Affairs. Faperika. Padjadjaran University. Bandung. 75-83.
Baehaki, A. and B.A. Rinto. 2011. Isolation of Bacteria and Proteases Karekterisasi Indralaya Swamp Land , South Sumatra. J. Technology and the Food Industry, XXII (1): 10-16.
Duc, L.H., H.A. Hong, T.M. Barbosa, A.O.Henriques, and S.M. Cutting. 2004. Characterization of Bacillus probiotics available for human use . J. Appl. Environ. Microbiol., 70 (4) : 2161-2171.
Feliatra, I. Efendi, and E. Suryadi. 2004 . Isolation and Identification of Probiotic Bacteria Tiger Grouper (Ephinephelus fuscogutatus) In Fish Feed Efficiency Efforts. Indonesian Journal of Natur, ISSN 1410-9379.
Hagstrom, A.J.F. Pinhassi, and U.L. Zweiefel. 2000. Biogeoghraphycal Marine Diversity Among Bacretioplankton . Aquatic Microbial Technology, 21 : 231-244.
Irianto, A. 2003. Probiotics Aquaculture. Gadjah Mada
University Press. Yogyakarta. 125 p.
Radjasa, K.O. 2006. Psychrotrophic Rapid Grouping
of Marine Bacteria Using Fragent Restriction Length Polymorphism Analysis of PCR-Amplified 16S. Internet.http://www.karnaradjasa.com.
Rume, I.M., A. Rantetondo, and G. Latama. 2012. Isolation of Intestinal Bacteria Probiotics Tiger Shrimp And Their Applications in Effort to Control the Vibrio harveyi infecting larvae Tiger Shrimp (Penaeus monodon Fabricius). Journal of Hasanuddin University.
Sarkono, Faturrahman, and Y. Sofyan. 2010. Isolation and identification of lactic acid bacteria from parent abalone (Haliotis asinina) as a potential probiotic candidates. Biotechnology, 7 : 99-106.
Susanti, V.H. 2003. Isolation and Characterization of
Protease from Bacillus subtilis 1012M15. University of March, Surakarta.
Wongsa, P. and P. Werukhamkul. 2007. Product Development and Technical Service, Biosolution International. Thailand : Bangkadi Industrial Park 134/4.
Yulvizar, C. 2013. Isolation and Identification of Probiotic Bacteria on Rastrelliger sp. Biospecies, 6 : 1-7.