Main Article Content

Abstract

The efficacy of zinc amino acid complex (ZnAA; AvailaZn®) on growth performance and immunity of Pangasius catfish (Pangasianodon hypophthalmus) was assigned in CRD with 4 treatments and 5 replications per treatment. The treatments were control with supplemental inorganic zinc sulfate (ZnSO4;100 mg/L Zn; T1), two treatments of 100 mg/L zinc at different proportions of ZnSO4:ZnAA 70:30 (T2) and 40:60 (T3) and a treatment of only ZnAA at 50 mg/L Zn (T4; 50% of control). Pangasius catfish with average weight of 209 g were stocked in 1,000 L fiber tanks at the density of 15 fish per tank and fed treatment diets 3% of body weight twice daily for three months. The results indicated that there were improved (P<0.05) growth performance in terms of body weight gain, average daily gain and specific growth rate of Pangasius catfish fed supplemental ZnAA at different levels compared to fish fed supplemental ZnSO4 (T1). The specific growth rates were 0.89, 1.20, 1.16 and 1.27 % in T1, T2, T3, and T4 respectively. Feed conversion ratio were not significantly different (P=0.08). Protein efficiency ratio in group of fifish fed ZnAA(T2,T3,T4) were significantly better (P<0.05) than inorganicZnSO4(T1).White blood cell count, serum protein, immunoglobulin (IgM), hemoglobin, and super oxide anion in fish fed supplemental ZnAA were better (P<0.05) than fish fed inorganic ZnSO4 (T1). Therefore, using zinc amino acid complex (AvailaZn®) in Pangasius catfish diets can enhance catfish growth performance (P<0.05) and supplemental zinc amino acid complex (AvailaZn) level in the catfish diet may be decreased by up to 50% of supplemental inorganic zinc levels. In conclusively, supplementation zinc amino acid complex (AvailaZn®) in Pangasius catfish diet is recommended at the level of 50 mg/L for ZnAA as the sole supplemental Zn source or 100 mg/L Zn in 70:30 proportion of ZnSO4:ZnAA.

Keywords

Growth performance; Immunity; Organic zinc; Pangasius catfish; Striped catfish; Zinc amino acid complex

Article Details

Author Biographies

Orapint Jintasataporn, Kasetsart University, Bangkok, Thailand

Department of Aquaculture, Faculty of Fisheries

Terry Ward, Eden Prairie, MN USA

Zinpro Corporation,

Supalug Kattakdad, Kasetsart University, Bangkok, Thailand

Department of Aquaculture, Faculty of Fisheries

References

  1. AOAC (Association of Official Analytical Chemists). 2000. Official Methods of Analysis, 15th Edition. Association of Official Analytical Chemists, Washington, D.C.
  2. Ashmead, H.D.W. 1992. The Roles of Amino Acid
  3. Chelates in Animal Nutrition. Noyes Publications, NJ, 479 pp.
  4. Blaxhall, P.C. and K.W. Daisley.1973. Routine haematological methods for use with fish blood. Journal of Fish Biology, (5):771–781.
  5. Dubois, M.K., K.A. Gilles, J.K. Hamilton, P.A. Rebers, and F. Smith. 1956. Colorimetric method for determination of sugars and related substance. Analytical Chemistry, 28:350-356.
  6. Gomes, E.F. and S. Kaushik. 1993. Effect of replacement of dietary inorganic zinc by zinc methionine on vegetable and animal protein utilization by rainbow trout. Fish Nutr. Prac.INRA, 61, 897–902.
  7. Hardy, R.W. and K.D. Shearer. 1985. Effect of dietary calcium phosphate and zinc supplementation on whole body zinc concentration of rainbow trout (Salmo gairdneri). Can. J. Fish. Aquat. Sci., 42, 181–184.
  8. Hidalgo, M.C., A. Exposito, J.M. Palma, and M.D.L. Higuera. 2002. Oxidative stress generated by dietary Zn-deficiency: studies in rainbow trout (Oncorhynchus mykiss). Inter.J. Biochem. Cell Biol., 34: 183–193.
  9. Lowry, O.H., N.J. Rosebrough, A.L. Farr, and R.J.Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193:265-275.
  10. NRC. 2011. Nutrient requirements of fish and shrimp.
  11. The National Academies Press, Washington,D.C. 376 p.
  12. Marcelo V.C. do C. e Sa., L.E. Pezzato, M.M.B.F. Lima, and P. de M. es Padilha. 2004. Optimum zinc supplementation level in Nile tilapia Oreochromis niloticus juveniles diets. Aquaculture, 238 : 385–401.
  13. O’Dell, B.L., J.D. Browning, and P.G. Reeves.1987. Zinc deficiency increases the osmotic fragility of rat erythrocytes. J. Nutr., 117,1883– 1889.
  14. Paripatananont, T. 2002. Snakehead and Pangasius
  15. catfish. In Nutrient requirements and feeding of finfish for aquaculture. CAB International.
  16. Scarpa, J. and D.M. Gatlin. 1992. Zinc supplementation of practical channel catfish diets. Aquaculture, 41: 31-36.