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Apr 26, 2024
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May 5, 2024
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Abstract

Carotenoid is one of nutrition factors which can improve red color pigmentation. The purpose of this study was to investigate the best type of carotene which can increase red pigment performance and to evaluate how long the effect should be retaining in order to maintain red color performance on leopard grouper seeds. This study consisted of 2 experiments; those were the effect of various kinds of carotene materials in feed addition and the effect of the termination of the addition of carotene materials in the feed toward red color performance on leopard grouper seed. The treatments given to the first experiment were the provision of various kinds of carotene materials which were type of A. Haematococcus pluvialis, B.Phaffiarhodozyma, C. Astax Oil, D. Control (without carotene materials) and the treatments to the second experiment are A. Leopard grouper seed – phaffia was still given additional Phaffia rodhozyma, B. Leopard grouper seed – haematococcus was still given additional Haematococcus pluvialis, C. leopard grouper seed (from larvae - phaffia / haematococcus) was terminated from carotene materials addition. The first experiment was applied from larva up to D60 seed and for the second experiment was the follow-up response from the first experiment conducted on D60 seed up to D150. Based on the study result, it indicates that carotene materials of Haematococcus pluvialis type gave the best color performance improvement (P value 4.71 x 10-7 < 0.05). The provision of carotene materials intake decreases color performance both visually and total contain of carotene (P value 5.97 x 10-5< 0.05) (Tabel 1). Carotenenoids should be continuously given as trigger to maintain red color performance on leopard grouper seed. There was protein band in the range of 82.1 – 84.2 kDa which was assumed as protein expression of astaxanthin.

Keywords

Carotenoid; Leopard grouper; Pigment; Red color

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Copyright (c) 2016 Aquacultura Indonesiana

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biographies

Daniar Kusumawati, Banjar Dinas Gondol, DesaPenyabangan,

Institute for Mariculture Research and Development

Ketut Maha Setiawati, Banjar Dinas Gondol, DesaPenyabangan,

Institute for Mariculture Research and Development

References

  1. Amiya, N., M. Amano, T. Yamanome, K. Yamamori,
  2. and A. Takahashi. 2008. Effects of Background Color on GnRH and MCH Levels in the Barfin Flounder Brain. General and Comparative Endocrinology, 155: 88-93.
  3. Bollag, D.M and S.J. Edelstein. 1991. Protein Methods. Departemen of Biochemistry. University of Geneva, Geneva, Switzerland
  4. Brock, T.D., M.T. Madigan, J.M. Martinko, and J.Parker. 1999. Biology of microorganisms. Prentice Hall. Englewood Cliffs : New Jersey. P: 550-561.
  5. Choubert, G. and T. Storebakken. 1989. Dose response to astaxanthin and canthaxanthin pigmentation of rainbow trout fed various dietary carotenoid concentrations. Aquaculture, 81 (1): 69-77.
  6. Del Campo, J.A. and M. García-González. 2007. Outdoor cultivation of microalgae for carotenoid production: current state and perspectives. Appl. Microbiol. Biotech., 74 : 1163–1174. doi: 10.1007/s00253-007-0844-9.
  7. Doolan, B.J., G.L. Allan, M.A. Booth, and P.L. Jones. 2008. Effect of carotenoids and background colour on the skin pigmentation of Australian snapper Pagrus auratus (Bloch & Schneider, 1801). Aquaculture Research, 39 : 1423–1433.
  8. Furr, H.C. and R.M. Clark. 1997. Intestinal absorption and tissue distribution of carotenoids. The Journal of Nutritional Biochemistr, 8 (7) : 364-377.
  9. Gouveia, L., P. Rema, O. Pereira, and J. Empis.
  10. Colouring Ornamental Fish (Cyprinus carpio and Carassius auratus) With Microalgal Biomass. Aquaculture Nutrition. Blackwell Publishing Ltd. P. 125-129.
  11. Hirschberg, J. 1999. Production of high-value compounds: carotenoids and vitamin E. Biotechnology, 10 : 186 – 191.
  12. Johnson, E.A. and M.J. Lewis. 1979. Astaxanthin Formation by the Yeast Phafia rhodozyma. Journal of General Microbiology, 115 :173- 183.
  13. Karlsson, A. 2001. Signaling For Color Change In
  14. Melanophore And A Bioseneor AP.lication. Linköpings University Medical Dissertations. Sweden. P. 53.
  15. Kelsh, R.N. 2004. Review: Pigment Gene Focus. Genetics and Evolution of Pigment Patterns in Fish. Pigment Cell Res., 17 : 326-336.
  16. Korzan, W.J., R.R. Robinson, S. Zhao, and R.D. Fernald. 2008. Color Change As Potential Behavioral Strategy. Hormones and Behavior, 54 : 463-470.
  17. Li, M., W. Wua, P. Zhou, F. Xie, Q. Zhou, and K. Mai. 2014. Comparison effect of dietary astaxanthin and Haematococcus pluvialis on growth performance, antioxidant status and immune response of large yellow croaker Pseudosciaena crocea. Aquaculture, 434 : 227 – 232.
  18. Nery, L.E.M and A.M.L. Castrucci. 1997. Pigment
  19. Cell Signalling For Physiological Color Change. Comparative Biochemistry And Physiology, 118A (4) : 1135-1144.
  20. Nickell, D.C. and N.R. Bromage. 1998. The effect of
  21. dietary lipid level on variation of flesh pigmentation in rainbow trout (Oncorhynchus mykiss). Aquaculture, 161 (1–4): 237-251.
  22. Olsen, R.E., A. Kiessling, J.E. Milley, N.W. Ross,and S.P. Lall. 2005. Effect of lipid source and bile salts in diet of Atlantic salmon, Salmo salar L., on astaxanthin blood levels. Aquaculture, 250 : 804– 812.
  23. Packer, L. 1992. Carotenoids. Academic Press, San
  24. Diego,California, USA.
  25. Sanderson, G.W. and S.O. Jolly. 1994. The value of
  26. Phaffia yeast as a feed ingredient for salmonid fish. Aquaculture, 124 : 193-200. Storebakken, T. and H.K. No. 1992. Pigmentation of
  27. rainbow trout. Aquaculture, 100 (1): 209-229.
  28. Sugama, K., Tridjoko, B. Slamet, S. Ismi, E. Setiadi, and S. Kawahara. 2001. Petunjuk teknis produksi benih ikan kerapu bebek, Cromileptes altivelis. Balai Riset Budidaya Laut Gondol. Pusat Riset dan Pengembangan Eksploitasi Laut dan Perikanan. Departemen Kelautan dan Perikanan serta Japan International Cooperation Agency. 40 p.
  29. Sheikhzadeh, N., I.K. Panchah, R. Asadpour, H. Tayefi-Nasrabadi, and H. Mahmoudi.2012. Effects of Haematococcus pluvialis in maternal diet on reproductive performance and egg quality in rainbow trout (Oncorhynchus mykiss). Animal Reproduction Science, 130: 119– 123.
  30. Sugie, A., Y. Terai, R. Ota, and N. Okada. 2004. The Evolution of Genes for Pigmentation in African Cichlid Fishes. Gene. Vol. 343.Elsevier. P. 337-346.
  31. Tanaka, Y., T. Katayama, K.L. Simpson, and C.O.Chichester. 1976. The Biosynthesis of Astaxanthin-XX : The Carotenoids in Marine Red Fish and the Metabolismof the Carotenoids in Sea Bream, Chrysophrys major Temminck and Schlegel. Bulletin of the Japanese Society of Scientific Fisheries, 42 (10): 1177-1182.
  32. Torrissen, O.J. 1989. Pigmentation of salmonids: interactions of astaxanthin and canthaxanthin on pigment deposition in rainbow trout. Aquaculture, 79 (1): 363-374.
  33. Yasir, I and J.G. Qin. 2010. Effect of Dietary Carotenoids on Skin Color and Pigments of False Clownfish, Amphiprion ocellaris, Cuvier. Journal of the World Aquaculture Society, 41 (3): 309 – 318.
  34. Yang, H., X. Mu, Du. Luo, Y. Hu, H. Song, C. Liu, and J. Luo. 2012. Sodium taurocholate, a novel effective feed‐additive for promoting absorption and pigmentation of astaxanthin in blood parrot (Cichlasoma synspilum ♀× Cichlasoma citrinellum ♂). Aquaculture, 350–353 : 42 – 45.
  35. Yi, X., F. Zhang, Xu. Wei, J. Li, W. Zhang, and K.
  36. Mai. 2014a. Effects of dietary lipid content on growth, body composition and pigmentation of large yellow croaker Larimichthys croceus. Aquaculture, 434: 355-361.
  37. Yi, X., W. Xu, H. Zhou, Y. Zhang, Y. Luo, W. Zhang, and K. Mai. 2014b. Effects of dietary astaxanthin and xanthophylls on the growth and skin pigmentation of large yellow croaker Larimichthys croceus. Aquaculture, 433 : 377–383.