Main Article Content
Abstract
Asian seabass (Lates calcarifer) aquaculture is a potential developing industry in Australia and Indonesia. As the aquaculture industry intensifies, the occurrence of significant diseases becomes more likely. Hence, it is important to find better methods for controlling diseases in this industry. Research on mammals and fish have previously demonstrated the potential benefits of dietary nucleotides to enhance immune function of the animal to combat diseases. However there is still very limited study of this subject in Asian seabass. This study aimed to investigate the role of dietary nucleotides on the growth and immune responses of Asian seabass. Juvenile Asian seabass with an average weight of 13.8±1.29 (mean ± SD) g were fed three different diets containing either Optimun® nucleotide or a mix of pure nucleotides at 2.5 g/kg feed (0.25%) and one basal diet (nucleotide free diet). Fish were fed at 3% BW per day for 28 days. Results showed that dietary nucleotides did not significantly effect the growth, survival rate, and immune responses of the fish. However Optimun® diet gave higher result in terms of growth, leucocrit level, lysozyme activity and respiratory burst activity of head kidney leucocyte compare to basal and the mix of pure nucleotide diets.
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Copyright (c) 2016 Aquacultura Indonesiana

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References
- Andrino, K.G.S., A.E. Serrano Jr, and V.L. Corre
- Jr. 2012. Effects of Dietary Nucleotides on
- the Immune Response and Growth of
- Juvenile Pacific White Shrimp Litopenaeus
- vannamei (Boone, 1931). Asian Fisheries
- Science, 25: 180-192.
- Barros, M.M., I.G. Guimaraes, L.E. Pezzato, R.O.
- Orsi, A.C.F. Junior, C.P. Teixeira, L.F.
- Fleuri, and C.R. Padovani. 2015. The
- effects of dietary nucleotide mixture on
- growth, performance, haematological and
- immunological parameters of Nile tilapia.
- Aquaculture Research, 46: 987-993.
- Bondad-Reantaso, M.G., R.P. Subasinghe, J.R.
- Arthur, K. Ogawa, S. Chinabut, R.
- Adlard, Z. Tan, and M. Shariff. 2005.
- Disease and health management in Asian
- aquaculture. Veterinary Parasitology, 132:
- -272.
- Burrells, C., P.D. Williams, and P.F. Forno. 2001a
- .
- Dietary nucleotides: a novel supplement in
- fish feeds 1. Effects on resistance to disease
- in salmonids. Aquaculture, 199 (1): 159-169.
- Burrells, C., P.D. Williams, P.J. Southgate, and
- S.L. Wadsworth. 2001b
- . Dietary nucleotides:
- a novel supplement in fish feeds 2. Effects on
- vaccination, salt water transfer, growth rates
- and physiology of Atlantic salmon (Salmo
- salar L.). Aquaculture, 199: 171-184.
- Cabello, F.C. 2006. Heavy use of prophylactic
- antibiotics in aquaculture: a growing problem
- for human and animal health and for the
- environment. Environmental Microbiology, 8
- (7): 1137-1144.
- Carver, J.D. and W.A. Walker. 1995. The role of
- nucleotides in human nutrition. Nutritional
- Biochemistry, 6: 58-72.
- Cheng, Z., A. Buentello, and D.M.I. Gatlin. 2011.
- Dietary nucleotide influence immune
- responses and intestinal morphology of red
- drum Sciaenops ocellatus. Fish & Shellfish
- Immunology, 30: 143-147.
- Ellis, A.E. 1990. Lysozyme activity. In T.C. Stolen,
- P.D. Fletcher, B.S. Anderson, B.S. Roberson,
- & W.B. Muiswinkel (Eds.), Technique in
- Fish Immunology (pp. 101-103). New Jersey,
- USA: SOS Publications.
- Fuchs, V.I., J. Schmidt, M.J. Slater, J. Zentek, B.H.
- Buck, and D. Steinhagen. 2015. The effect
- of supplementation with polysaccharides,
- nucleotides, acidifiers and Bacillus strains in
- fish meal and soy bean based diets on growth
- performance in juvenile turbot (Scophthalmus
- maximus). Aquaculture, 437: 243-251.
- Gatlin III, D.M. and P. Li. 2007. Nucleotides.
- Trowbridge, United Kingdom: Cromwell
- Press, Trowbridge UK.
- Kenari, A.A., N. Mahmoudi, M. Soltani, and S.A.
- Kenari. 2013. Dietary nucleotide
- supplements influence the growth, haemato-
- immunological parameters and stress
- responses in endangered Caspian brown trout
- (Salmo trutta caspius Kessler, 1887).
- Aquaculture Nutrition, 19: 54-63.
- Li, P., Burr, G.S., J. Goff, K.W. Whiteman, K.B.
- Davis, R.R. Vega, W.H. Neil, and D.M.
- Gatlin III. 2005. A preliminary study on the
- effects of dietary supplementation of brewers
- yeast and nucleotides, singularly or in
- combination, on juvenile red drum
- (Sciaenops ocellatus). Aquaculture Research,
- : 1120-1127.
- Li, P. and D.M. Gatlin III. 2007. Dietary
- Supplementation of a Purified Nucleotide
- Mixture Transiently Enhanced Growth and
- Feed Utilization of Juvenile Red Drum,
- Sciaenops ocellatus. Journal of The World
- Aquaculture Society, 38 (2): 281-286.
- Lin, Y-H., H. Wang, and S-Y. Shiau. 2009. Dietary
- nucleotide supplementation enhances growth
- and immune responses of grouper,
- Epinephelus malabaricus. Aquaculture
- Nutrition, 15: 117-122.
- Low, C., S. Wadsworth, C. Burrells, and C.J.
- Secombes. 2003. Expression of immune
- genes in turbot (Scophthalmus maximus) fed
- a nucleotide-supplemented diet. Aquaculture,
- , 23-40.
- Oliva-Teles, A. and P. Goncalves. 2001. Partial
- replacement of fishmeal by brewers yeast
- (Saccaromyces cerevisae) in diets for seabass
- (Dicentrarchus labrax) juveniles.
- Aquaculture, 202, 269-278.
- Peng, M., W. Xu, Q. Ai, K. Mai, Z. Liufu, and K.
- Zhang. 2013. Effects of nucleotide
- supplementation on growth, immune
- responses and intestinal morphology in
- juvenile turbot fed diets with graded levels of
- soybean meal (Scophthalmus maximus L.).
- Aquaculture, 392-395: 51-58.
- Russo, R. and R.P.E. Yanong. 2006. Dietary Beta-
- Glucans and Nucleotides Enhance Resistance of Red-Tail Black Shark (Epalzeorhynchos bicolor, fam. Cyprinidae) to Streptococcus iniae Infection. World Aquaculture Society,
- (3): 298-306.
- Sakai, M. 1999. Current research status of fish
- immunostimulants. Aquaculture, 172 (1): 63-92.
- Sakai, M., K. Taniguchi, K. Mamoto, H. Ogawa, and M. Tabata. 2001. Immunostimulant effects of nucleotide isolated from yeast RNA on carp, Cyprinus carpio L. Journal of Fish Diseases, 24 (8): 433-438.
- Secombes, C.J. 1990. Isolation of salmonid macophages and analysis of their killing activity. Techniques in fish immunology, 1:137-154.
- Shiau, S.-Y., J. Gabaudan, and Y.-H. Lin. 2015. Dietary nucleotide supplementation enhances immune responses and survival to Streptococcus iniae in hybrid tilapia fed diet
- containing low fish meal. Aquaculture Reports, 2: 77-81.
- Siwicki, A., D. Anderson, and G. Rumsey. 1994.
- Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Veterinary Immunology and Immunopathology, 41: 125-139.
- Tahmasebi-Kohyani, A., S. Keyvanshokooh, A.Nematollahi, N. Mahmoudi, and H. Pasha-Zanoosi. 2011. Dietary administration of nucleotides to enhance growth, humoral immune responses, and disease resistance of the rainbow trout (Oncorhynchus mykiss) fingerlings. Fish & Shellfish Immunology, 30:189-193.
- Tahmasebi-Kohyani, A., S. Keyvanshokooh, A.Nematollahi, N. Mahmoudi, and H. Pasha-Zanoosi. 2012. Effects of dietary nucleotides supplementation on rainbow trout (Oncorhynchus mykiss) performance and acute stress response. Fish Physiology and Biochemistry, 38: 431-440.
- Welker, T.L., C. Lim, M. Yildirim-Aksoy, and P.H.
- Klesius. 2011. Effects of dietary supplementation of a purified nucleotide mixture on immune function and disease and stress resistance in channel catfish, Ictalurus punctatus. Aquaculture Research, 42: 1878- 1889.
- Yousefi, M., B. Abtahi, and A.A. Kenari. 2012. Hematological, serum biochemical parameters, and physiological responses to acute stress of Beluga sturgeon (Huso huso, Linnaeus 1785) juveniles fed dietary nucleotide. Comparative Clinical Pathology, 21: 1043-1048.
References
Andrino, K.G.S., A.E. Serrano Jr, and V.L. Corre
Jr. 2012. Effects of Dietary Nucleotides on
the Immune Response and Growth of
Juvenile Pacific White Shrimp Litopenaeus
vannamei (Boone, 1931). Asian Fisheries
Science, 25: 180-192.
Barros, M.M., I.G. Guimaraes, L.E. Pezzato, R.O.
Orsi, A.C.F. Junior, C.P. Teixeira, L.F.
Fleuri, and C.R. Padovani. 2015. The
effects of dietary nucleotide mixture on
growth, performance, haematological and
immunological parameters of Nile tilapia.
Aquaculture Research, 46: 987-993.
Bondad-Reantaso, M.G., R.P. Subasinghe, J.R.
Arthur, K. Ogawa, S. Chinabut, R.
Adlard, Z. Tan, and M. Shariff. 2005.
Disease and health management in Asian
aquaculture. Veterinary Parasitology, 132:
-272.
Burrells, C., P.D. Williams, and P.F. Forno. 2001a
.
Dietary nucleotides: a novel supplement in
fish feeds 1. Effects on resistance to disease
in salmonids. Aquaculture, 199 (1): 159-169.
Burrells, C., P.D. Williams, P.J. Southgate, and
S.L. Wadsworth. 2001b
. Dietary nucleotides:
a novel supplement in fish feeds 2. Effects on
vaccination, salt water transfer, growth rates
and physiology of Atlantic salmon (Salmo
salar L.). Aquaculture, 199: 171-184.
Cabello, F.C. 2006. Heavy use of prophylactic
antibiotics in aquaculture: a growing problem
for human and animal health and for the
environment. Environmental Microbiology, 8
(7): 1137-1144.
Carver, J.D. and W.A. Walker. 1995. The role of
nucleotides in human nutrition. Nutritional
Biochemistry, 6: 58-72.
Cheng, Z., A. Buentello, and D.M.I. Gatlin. 2011.
Dietary nucleotide influence immune
responses and intestinal morphology of red
drum Sciaenops ocellatus. Fish & Shellfish
Immunology, 30: 143-147.
Ellis, A.E. 1990. Lysozyme activity. In T.C. Stolen,
P.D. Fletcher, B.S. Anderson, B.S. Roberson,
& W.B. Muiswinkel (Eds.), Technique in
Fish Immunology (pp. 101-103). New Jersey,
USA: SOS Publications.
Fuchs, V.I., J. Schmidt, M.J. Slater, J. Zentek, B.H.
Buck, and D. Steinhagen. 2015. The effect
of supplementation with polysaccharides,
nucleotides, acidifiers and Bacillus strains in
fish meal and soy bean based diets on growth
performance in juvenile turbot (Scophthalmus
maximus). Aquaculture, 437: 243-251.
Gatlin III, D.M. and P. Li. 2007. Nucleotides.
Trowbridge, United Kingdom: Cromwell
Press, Trowbridge UK.
Kenari, A.A., N. Mahmoudi, M. Soltani, and S.A.
Kenari. 2013. Dietary nucleotide
supplements influence the growth, haemato-
immunological parameters and stress
responses in endangered Caspian brown trout
(Salmo trutta caspius Kessler, 1887).
Aquaculture Nutrition, 19: 54-63.
Li, P., Burr, G.S., J. Goff, K.W. Whiteman, K.B.
Davis, R.R. Vega, W.H. Neil, and D.M.
Gatlin III. 2005. A preliminary study on the
effects of dietary supplementation of brewers
yeast and nucleotides, singularly or in
combination, on juvenile red drum
(Sciaenops ocellatus). Aquaculture Research,
: 1120-1127.
Li, P. and D.M. Gatlin III. 2007. Dietary
Supplementation of a Purified Nucleotide
Mixture Transiently Enhanced Growth and
Feed Utilization of Juvenile Red Drum,
Sciaenops ocellatus. Journal of The World
Aquaculture Society, 38 (2): 281-286.
Lin, Y-H., H. Wang, and S-Y. Shiau. 2009. Dietary
nucleotide supplementation enhances growth
and immune responses of grouper,
Epinephelus malabaricus. Aquaculture
Nutrition, 15: 117-122.
Low, C., S. Wadsworth, C. Burrells, and C.J.
Secombes. 2003. Expression of immune
genes in turbot (Scophthalmus maximus) fed
a nucleotide-supplemented diet. Aquaculture,
, 23-40.
Oliva-Teles, A. and P. Goncalves. 2001. Partial
replacement of fishmeal by brewers yeast
(Saccaromyces cerevisae) in diets for seabass
(Dicentrarchus labrax) juveniles.
Aquaculture, 202, 269-278.
Peng, M., W. Xu, Q. Ai, K. Mai, Z. Liufu, and K.
Zhang. 2013. Effects of nucleotide
supplementation on growth, immune
responses and intestinal morphology in
juvenile turbot fed diets with graded levels of
soybean meal (Scophthalmus maximus L.).
Aquaculture, 392-395: 51-58.
Russo, R. and R.P.E. Yanong. 2006. Dietary Beta-
Glucans and Nucleotides Enhance Resistance of Red-Tail Black Shark (Epalzeorhynchos bicolor, fam. Cyprinidae) to Streptococcus iniae Infection. World Aquaculture Society,
(3): 298-306.
Sakai, M. 1999. Current research status of fish
immunostimulants. Aquaculture, 172 (1): 63-92.
Sakai, M., K. Taniguchi, K. Mamoto, H. Ogawa, and M. Tabata. 2001. Immunostimulant effects of nucleotide isolated from yeast RNA on carp, Cyprinus carpio L. Journal of Fish Diseases, 24 (8): 433-438.
Secombes, C.J. 1990. Isolation of salmonid macophages and analysis of their killing activity. Techniques in fish immunology, 1:137-154.
Shiau, S.-Y., J. Gabaudan, and Y.-H. Lin. 2015. Dietary nucleotide supplementation enhances immune responses and survival to Streptococcus iniae in hybrid tilapia fed diet
containing low fish meal. Aquaculture Reports, 2: 77-81.
Siwicki, A., D. Anderson, and G. Rumsey. 1994.
Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Veterinary Immunology and Immunopathology, 41: 125-139.
Tahmasebi-Kohyani, A., S. Keyvanshokooh, A.Nematollahi, N. Mahmoudi, and H. Pasha-Zanoosi. 2011. Dietary administration of nucleotides to enhance growth, humoral immune responses, and disease resistance of the rainbow trout (Oncorhynchus mykiss) fingerlings. Fish & Shellfish Immunology, 30:189-193.
Tahmasebi-Kohyani, A., S. Keyvanshokooh, A.Nematollahi, N. Mahmoudi, and H. Pasha-Zanoosi. 2012. Effects of dietary nucleotides supplementation on rainbow trout (Oncorhynchus mykiss) performance and acute stress response. Fish Physiology and Biochemistry, 38: 431-440.
Welker, T.L., C. Lim, M. Yildirim-Aksoy, and P.H.
Klesius. 2011. Effects of dietary supplementation of a purified nucleotide mixture on immune function and disease and stress resistance in channel catfish, Ictalurus punctatus. Aquaculture Research, 42: 1878- 1889.
Yousefi, M., B. Abtahi, and A.A. Kenari. 2012. Hematological, serum biochemical parameters, and physiological responses to acute stress of Beluga sturgeon (Huso huso, Linnaeus 1785) juveniles fed dietary nucleotide. Comparative Clinical Pathology, 21: 1043-1048.
